3,302 Views
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09:48 min
July 27, 2022
DOI:
10.3791/64215-v
本协议概述了一种在顶端肠模型中利用荧光花黄来确定肠道通透性的方法。该方法可用于确定模拟炎症性肠病(如坏死性小肠结肠炎)的肠样肠的细胞旁通透性。
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Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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Cite this Article
Liebe, H., Schlegel, C., Cai, X., Golubkova, A., Leiva, T., Berry, W. L., Hunter, C. J. Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model. J. Vis. Exp. (185), e64215, doi:10.3791/64215 (2022).
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