Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells

Malcolm Lim; Nicholas Fletcher; Amy McCart Reed; Melanie Flint; Kristofer Thurecht; Jodi Saunus; Sunil R. Lakhani

doi:10.3791/64216

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells

通过颈内动脉注射癌细胞模拟脑转移

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10:01 min

August 2, 2022

DOI: 10.3791/64216-v

Malcolm Lim¹, Nicholas Fletcher², Amy McCart Reed¹, Melanie Flint³, Kristofer Thurecht², Jodi Saunus*^1,4, Sunil R. Lakhani*^1,5

¹UQ Centre for Clinical Research,The University of Queensland, ²Centre for Advanced Imaging, Australian Institute for Bioengineering and Nanotechnology and ARC Training Centre for Innovation in Biomedical Imaging Technology,The University of Queensland, ³School of Biomedical Sciences,The University of Queensland, ⁴Mater Research and Mater Research Institute,The University of Queensland, ⁵Pathology Queensland,Royal Brisbane Women's Hospital

Please note that some of the translations on this page are AI generated. Click here for the English version.

脑转移是癌症患者严重发病和死亡的原因。大多数脑转移小鼠模型因全身转移而复杂化，混淆死亡率和治疗干预结果的分析。这里介绍的是颈动脉内注射癌细胞的方案，该方案产生一致的颅内肿瘤和最小的全身性肿瘤。

那么为什么这个协议很重要呢？好吧，脑转移瘤的治疗非常具有挑战性，并且该协议产生了具有最小颅外肿瘤的临床相关小鼠模型。该技术可以客观分析颅内肿瘤的药物分布和反应。

该技术的主要优点是该模型具有高度可重复性，并且在注射后相对较短的时间内发展脑转移。动物之间的疾病进展相似，使研究人员更容易标准化实验、治疗时间表和终点。我对初学者的建议是练习使用镊子和注射器，同时通过解剖显微镜观察，熟悉颈部周围的解剖标志并在兽医在场的情况下练习协议。

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