Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Magdalena Zemelka-Wiacek; Monika Majewska-Szczepanik; Pawel Gajdanowicz; Marian Szczepanik

doi:10.3791/64329

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

接触超敏反应作为过敏性接触性皮炎的小鼠模型

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08:25 min

September 26, 2022

DOI: 10.3791/64329-v

Magdalena Zemelka-Wiacek¹, Monika Majewska-Szczepanik², Pawel Gajdanowicz¹, Marian Szczepanik³

¹Department of Clinical Immunology,Wroclaw Medical University, ²Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences,Jagiellonian University Medical College, ³Chair of Biomedical Sciences, Faculty of Health Sciences,Jagiellonian University Medical College

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接触性超敏反应（CHS）是过敏性接触性皮炎（ACD）的小鼠实验模型。CHS基于通过涂上胸部和腹部的剃须皮肤对反应性半抗原的致敏，随后用稀释的半抗原进行耳朵皮肤激发，引起肿胀反应，以各种方式评估。

这里介绍的客观实验室方法可能有助于研究小鼠的接触性超敏反应，其类似于人类的过敏性接触性皮炎。接触性超敏反应不仅可以通过耳水肿来测量，还可以通过各种实验室测试来测量，这些测试可以研究参与该反应的细胞机制。接触性更强的超敏反应模型可以测试各种环境因素和新物质，以显示测试因素是否调节T细胞依赖性免疫反应，可用于实施新疗法。

首先用剃须刀片刮掉老鼠的皮肤并标记爪子。在诱导接触性超敏反应（CHS）前六小时，用水涂抹灰色肥皂，并在小鼠的胸部和腹部剃掉两厘米见方的区域。在同一天，通过在先前剃光的斑点上施用150微升新鲜制备的5%半抗原来使小鼠敏感。

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