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A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
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发育生物学
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JoVE 杂志 发育生物学
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

DOI:
10.3791/64582-v

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08:19 min

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December 16, 2022

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, , , , , , , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Département de Biologie,École Normale Supérieure de Lyon, 4Department of Critical Care Medicine,University of Pittsburgh

Chapters

  • 00:05Introduction
  • 01:05Fetal Echocardiogram
  • 01:46Necropsy
  • 02:41Episcopic Confocal Microscopy (ECM)
  • 04:01Three-Dimensional (3D) Reconstruction
  • 06:14Results: Analyzing Cardiac Anomalies Using Echo and ECM
  • 07:19Conclusion

Summary

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This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

Tags

Fetal MouseCongenital Heart DefectsEchocardiographyNecropsyConfocal MicroscopyAnatomical ChangesStructural Birth DefectsUltrasoundColor DopplerSample PreparationLaser Confocal ImagingImage Acquisition

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