Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

Maodi Wu; Yang Liu; Yi Zhao; Yuyao Zhang; Liansi Huang; Qijun Du; Ting Zhang; Zhihui Zhong; Han Luo; Kai Xiao

doi:10.3791/64623

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

间变性甲状腺癌和头颈部鳞状细胞癌患者来源异种移植模型的建立与表征

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06:08 min

June 2, 2023

DOI: 10.3791/64623-v

Maodi Wu^1,3, Yang Liu², Yi Zhao³, Yuyao Zhang⁴, Liansi Huang³, Qijun Du³, Ting Zhang^1,3, Zhihui Zhong^1,3, Han Luo², Kai Xiao^1,3

¹Precision Medicine Research Center, Sichuan Provincial Key Laboratory of Precision Medicine, National Clinical Research Center for Geriatrics, West China Hospital,Sichuan University, ²Department of Thyroid Surgery, West China Hospital,Sichuan University, ³Sichuan Kangcheng Biotech Co., ⁴Sichuan Cancer Hospital

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由于PDX模型正在迅速成为转化肿瘤学领域的标准，因此本协议建立并表征了间变性甲状腺癌（ATC）和头颈部鳞状细胞癌（HNSCC）的患者来源异种移植（PDX）模型。

该协议有助于建立ATC和HNSCC PDX模型，以更好地反映肿瘤异质性，分子多样性并预测临床结果。该技术操作简单，成功率高，也适用于其他PDX型号。该技术可向抗癌药物敏感性筛查延伸，指导癌症患者的个性化治疗。

从未执行过此技术的个人可能会遇到一些困难，建议注意方案的每个细节。在含有无菌HTK溶液的50毫米离心管中获得ATC和HNSCC肿瘤样品后，用75%酒精消毒。使用无菌眼科镊子将肿瘤组织转移到装有盐水的六厘米培养皿中，并使用刀片将它们切成小块。

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