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Immunology and Infection

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JoVE Journal Immunology and Infection
Påvisning af polyfunktionelle T-celler hos børn vaccineret med japansk encephalitisvaccine via flowcytometriteknikken
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Påvisning af polyfunktionelle T-celler hos børn vaccineret med japansk encephalitisvaccine via flowcytometriteknikken

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Den nuværende protokol kombinerer ex vivo-stimulering og flowcytometri til analyse af polyfunktionelle T-celleprofiler (TPF) i mononukleære celler (PBMC'er) i perifert blod mononukleære celler (PBMC'er) hos japanske encephalitisvirus (JEV)-vaccinerede børn. Detektionsmetoden og flowcytometrifarveskemaet for JEV-specifikke TPF'er blev testet for at give en reference til lignende undersøgelser.

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Immunologi og infektion udgave 187 Polyfunktionelle T-celler japansk encephalitis vaccination flowcytometri
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