Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model

Meaghan  J. Griffiths; Jordan N. Higgins; Fiona L. Cousins; Lauren R. Alesi; Amy L. Winship; Karla J. Hutt

doi:10.3791/64763

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model

研究卵巢切除小鼠模型中子宫对妊娠建立的贡献的方法

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06:49 min

April 7, 2023

DOI: 10.3791/64763-v

Meaghan J. Griffiths*^1,2, Jordan N. Higgins*¹, Fiona L. Cousins³, Lauren R. Alesi¹, Amy L. Winship*¹, Karla J. Hutt*¹

¹Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology,Monash University, ²Gynaecology Research Centre, Royal Women’s Hospital and Department of Obstetrics and Gynaecology,University of Melbourne, ³The Ritchie Centre, Hudson Institute for Medical Research and Department of Obstetrics and Gynaecology,Monash University

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怀孕的建立是一个动态的过程，涉及复杂的胚胎和子宫串扰。母体子宫环境对这些过程的确切贡献仍然是一个活跃的研究领域。在这里，提供了详细的协议来帮助设计体内动物模型来解决这些研究问题。

因此，很难区分子宫和卵巢荷尔蒙对怀孕的影响，因为两者密切相关。因此，该协议允许我们直接检查子宫贡献。虽然其中一些技术（如卵巢切除术）具有很强的侵入性，但它们在练习和训练后相对简单，这使得它们适合刚接触小鼠模型的研究人员。

因此，这种方法可以用来理解HPG轴的更广泛调节，因为卵巢和子宫在该轴的调节中都起着重要作用。如果您以前从未执行过这些技术，请咨询您研究所的兽医团队以获得培训和支持。首先，在脊柱驼背下方的凹痕处剃出一小块区域后，轻轻地将其轻拍在眼睛上，将眼睛润滑剂大量涂抹在麻醉的小鼠上。

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撤稿问题 194 生育怀孕子宫卵巢切除术容受性蜕膜化