Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Janko Kajtez; Carmen Radeke; Johan Ulrik Lind; Jenny Emn&#233;us

doi:10.3791/65158

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

微凝胶-细胞外基质复合材料支持人类神经结构的嵌入式3D打印

Full Text

1,898 Views

07:48 min

May 5, 2023

DOI: 10.3791/65158-v

Janko Kajtez¹, Carmen Radeke², Johan Ulrik Lind², Jenny Emnéus³

¹Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),University of Copenhagen, ²Department of Health Technology (DTU Health Tech),Technical University of Denmark, ³Department of Biotechnology and Biomedicine (DTU Bioengineering),Technical University of Denmark

Please note that some of the translations on this page are AI generated. Click here for the English version.

这项工作描述了在自修复可退火颗粒 - 细胞外基质复合材料中自由形式嵌入神经干细胞的协议。该协议能够以高保真度对互连的人类神经组织结构进行可编程模式化。

SHAPE复合材料中的嵌入式3D打印克服了传统的生物制造限制，能够对类似于天然细胞外环境的水凝胶内的机械敏感组织进行准确的图案化。SHAPE工具箱依赖于模块化生物材料设计，可以轻松更改打印载体配方。它还利用低成本材料和可访问的设备，以便其他研究小组进行简单的调整。

这种方法可用于创建空间定义的人类神经组织模型，以检查帕金森氏症等神经退行性疾病的神经元发育和通信。首先在超纯水中以每毫升2毫克的浓度制备碳酸钙溶液。将其与海藻酸盐溶液以相等的比例混合，并在室温下以650RPM磁力搅拌一小时。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

生物工程第195期