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DOI: 10.3791/65501-v
Amy L. Roda1, Gary Steck2, Thomas Fezza3, Todd Shelly3, Rita Duncan4, Nicholas Manoukis5, Lori Carvalho5, Abbie Fox6, Paul Kendra7, Daniel Carrillo4
1Animal Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Science and Technology (S&T) Miami,United States Department of Agriculture (USDA), 2Division of Plant Industry,Florida Department of Agriculture and Consumer Services, 3APHIS, PPQ, S&T,USDA, 4IFAS, Tropical Research and Education Center,University of Florida, 5Agricultural Research Service (ARS), Pacific Basin Agricultural Research Center,USDA, 6APHIS, PPQ, Field Operations,USDA, 7ARS, Subtropical Horticulture Research Station,USDA
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This study addresses the challenge of detecting tephritid fruit fly larvae in fruit, aiming to develop a faster and more accurate field method. The researchers highlight that processing fruit by mushing and sieving is more effective than traditional cutting and visual inspection, especially in emergency situations.
在田间增加对未成熟果蝇的检测可以引发及时的努力,以消除这些破坏性害虫的种群。与手工切割和目视检查相比,将寄主水果糊在袋子中并将果肉通过一系列筛子时,检测晚龄幼虫更快、更准确。
我们正在开发一种寻找蝇蝇幼虫的方法,该方法比目前切割和目视检查每个水果的方法更快、更准确。我们想开发一种简单的技术,可以在田间用于处理大量水果。这项研究的一个挑战是找到感染率低的水果,这可能代表了我们在紧急情况或根除计划中看到的情况。
由于我们无法看到果实,我们使用方法以少量幼虫人为感染关键宿主。在田间和实验室试验中,糊化和筛分果肉所需的时间更少,发现的幼虫比切割水果多。将果肉漂浮在红糖水溶液中有助于检测早期的龄幼虫。
对重要绒毛蚴宿主的果肉进行捣碎和筛分可能会增加在紧急项目期间检测到幼虫的可能性。我们将继续优化协议,以确定不同种类的受管制果蝇的最佳筛子尺寸,并建立水果类型、水果数量等标准,这将有助于决定是否有必要使用幼虫破坏方法或标准水果切割。
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