Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self&#45;Assembling Peptide Matrix

Rosario Perez-Pedroza; Manola Moretti; Charlotte A. E. Hauser

doi:10.3791/66060

JoVE Journal
Bioengineering
Author Produced

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JoVE Journal Bioengineering

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

在超短自组装肽基质中制备和表征来自 SW1222 细胞系的结直肠癌类器官

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10:23 min

May 3, 2024

DOI: 10.3791/66060-v

Rosario Perez-Pedroza^1,2, Manola Moretti^1,2,3, Charlotte A. E. Hauser^1,2,3

¹Laboratory for Nanomedicine, Biological & Environmental Science & Engineering,King Abdullah University of Science and Technology, ²Computational Bioscience Research Center,King Abdullah University of Science and Technology, ³Red Sea Research Center,King Abdullah University of Science and Technology

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该方案旨在通过免疫染色评估生物功能自组装肽的细胞粘附、类器官形态和基因表达。我们将使用结直肠癌细胞系提供一种经济高效的方法来获取用于强化测试的类器官。

我们的研究旨在评估超短自组装肽作为结直肠癌类器官培养物的基质。我们解决了有关类器官形态、活力、增殖、粘附和生物功能化影响的问题。目标是优化肽基质组成以实现有效的类器官生长，并为再生医学做出贡献。

我们领域的尖端技术包括用于精确有机制造的 3D 生物打印、用于动态培养环境的微流体平台，以及原子力显微镜和多光子显微镜等先进的成像工具。CRISPR-Cas9 基因编辑可优化基因修饰，而单细胞 RNA 测序可提供深入的分子见解。目前的实验挑战包括增强类器官的可重复性、优化更大结构的血管化以及模拟复杂的组织结构。

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