Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Our group has been dedicated to basic and translational research in organ fibrosis. With a recent focus on developing early diagnostic approaches, and precision therapies for pulmonary fibrosis. We developed a micro CT-based unilateral ventilation lung volumetric approach, enabling precise pulmonary fibrosis in variation. Potentially transforming preclinical intervention assessment.

Unilateral bleomycin installation induces precise lifelong injury in mice, ensuring comparable pre-treatment between growths. Quantify while micro CT for competency intervention evaluation. Our guru will develop AI assisted automatic fibrotic lesion segmentation algorithms to enhance quantity assessment accuracy.

[Instructor] To begin launch the 3D Slicer program to begin post-processing for unilateral selective lung segmentation and volume analysis. Click load to import imaging data in DICOM format. Display the axial, corneal and sagittal images in the main interface. Adjust sagittal view parameters to show hidden options. Then choose rename current volume and enter new name. In the segment editor click add to create a segmentation for the right lung. Adjust threshold range to minus 1000 and minus 350 Hounsfield Units and enable use for masking. Now use the paint tool with sphere brush to segment the desired lung regions. Select the island tool and choose keep selected island. Highlight the right lung, then click show 3D to display the 3D model in the visualization panel. In the terminology panel, change the 3D model's color to blue. After repeating the steps to segment the left lung, process the axial chest CT images to calculate ventilated lung volume. Then generate a 3D model. Now change the 3D model color of the left lung to yellow. Set the opacity of the right lung segmentation to 0.2. Capture screenshots of 3D view or slice images, and save them by clicking on annotation screenshot. Selecting image format, then modifying the save path. Use the segment statistics module to calculate lung volume in cubic millimeters, and analyze normal aerated regions. The left lung volume in the bleomycin treated group was significantly reduced compared to the saline treated control group. Collagen deposition in the lung tissue, indicated by Masson's trichrome staining, was significantly elevated in the bleomycin treated group.