Studying Interactions between Myeloid Cells and CAR T Cells <em>In Vitro</em> and <em>In Vivo</em>

Kun Yun; R. Leo Sakemura; Truc N. Huynh; Brooke Kimball; Elizabeth Siegler; Saad S. Kenderian

doi:10.3791/68013

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo

研究骨髓细胞和 CAR T 细胞在体外和体内的相互作用

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05:14 min

July 25, 2025

DOI: 10.3791/68013-v

Kun Yun^1,2,3, R. Leo Sakemura^1,4, Truc N. Huynh^1,4, Brooke Kimball^1,4, Elizabeth Siegler^1,4, Saad S. Kenderian^1,2,3,4,5

¹T Cell Engineering,Mayo Clinic, ²Department of Molecular Medicine,Mayo Clinic, ³Mayo Clinic Graduate School of Biomedical Sciences, ⁴Division of Hematology,Mayo Clinic, ⁵Department of Immunology,Mayo Clinic

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鉴于可用于研究人 CART 和髓系细胞之间相互作用的异种移植模型有限，我们建立了体外和体内模型来了解人巨噬细胞对 CART 细胞的影响。研究结果有可能被推广以评估巨噬细胞在肿瘤微环境中的作用并测试巨噬细胞靶向免疫疗法。

我们的研究提供了简化的体外和体内模型，以了解人免疫抑制巨噬细胞在套细胞淋巴瘤背景下对 CA 19 抗肿瘤活性的影响。在体内研究人类巨噬细胞和单核细胞最常用的模型是将造血干细胞植入根除的免疫缺陷小鼠体内。用人造血干细胞植入免疫缺陷小鼠以研究小鼠中的人髓系细胞可能既耗时又昂贵。

植入效率也可能受到限制。我们建立了更简化的体外和体内模型来研究人巨噬细胞、CAR T 和肿瘤细胞之间的相互作用。它也可用于测试其他巨噬细胞靶向免疫疗法。

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