A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA<sub>2</sub>

Zhuoyan Zeng; Mingsi Wei; Kaixin Zheng; Zimu Zhou; Shuhao Zhang; Yanglin Guo; Yuri N. Utkin; Ruben K. Dagda; Edward S. Gasanoff

doi:10.3791/68594

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA₂

用于研究磷脂酰肌醇磷酸基团对毒液 PLA2 膜致性作用影响的脂质体膜通透性测定

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September 26, 2025

DOI: 10.3791/68594-v

Zhuoyan Zeng¹, Mingsi Wei¹, Kaixin Zheng¹, Zimu Zhou¹, Shuhao Zhang¹, Yanglin Guo¹, Yuri N. Utkin², Ruben K. Dagda³, Edward S. Gasanoff^1,4

¹Advanced STEM Research Center,Beijing Chaoyang KaiWen Academy, ²Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, ³Department of Pharmacology,University of Nevada Reno, ⁴Belozersky Institute of Physico-Chemical Biology,M. V. Lomonosov Moscow State University

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我们提出了一种经济高效的方法来评估脂质体膜通透性，其灵敏度和分辨率优于传统技术。使用一种新型封装配体取代反应，该技术检测磷脂酶_{A 2} 诱导的模型膜变化。这种易于与标准分析集成，可提高研究膜生物物理和毒素相互作用的分辨率。

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