跨物种转录组的全面空间剖析 通过 Stereo-seq
1.4K 次观看
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10:22 分钟
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2025年10月31日
DOI :10.3791/68619-v
2025年10月31日
•1.4K 次观看
Anna K. Casasent*1 , Danielle L. Stolley*1 , Basant T. Gamal*2 , Arnold Dahay2 , Samuel Mok2 , Lidia Rocha3 , Vincent Li3 , Arizona T. Nguyen3 , Boyu Zhang4 , Clay T. Brasuell4 , Erika J. Thompson4 , Thomas Huynh3 , Jared K. Burks1 , Sammy Ferri-Borgogno2
1Department of Hematopoietic Biology and Malignancies, The University of Texas MD Anderson Cancer Center, 2Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, 3Department of Veterinary Medicine & Surgery, University of Texas MD Anderson Cancer Center, 4Department of Genetics, The University of Texas MD Anderson Cancer Center
* These authors contributed equally
本文介绍了一种利用Stereo-seq对福尔马林固定石蜡包埋组织切片中的宿主、病毒、真菌及微生物组RNA进行全方位空间转录组分析的实验方案，该技术可在保留组织结构的同时实现多种转录组的高分辨率定位。
Chapters in this video
0:00
Introduction
0:58
Stereo-Seq Random Oligonucleotide Capture Chip (N-Chip) Preparation
3:06
Nuclear Imaging and ssDNA Staining of the FFPE Tissue Sections
5:08
Decrosslinking Process of the Stained FFPE Sections
7:43
Collection of the cDNA Release Mix
8:56
Results
9:36
Conclusion
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