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Chapters
- 00:00Title
- 00:14Introduction
- 01:00Preparing Tools and Reagents
- 05:03Dissecting Midbrain of P0-P2 Rodent
- 09:10Triturating Brain Segments
- 10:49Plating Dissociated Neurons
- 12:53Primary Dissociated Midbrain Dopamine Cultures
- 13:28Conclusion
Primary dissociated midbrain dopamine cell cultures allow for the study of presynaptic characteristics of dopamine neurons. They can be used to monitor real-time dopamine release kinetics and protein/mRNA levels of regulators of dopamine exocytosis. Here, we show you how to generate these cultures from rodent neonates.
Tags
Primary Cell CulturesDopamine NeuronsPresynaptic CharacteristicsDopamine Release KineticsCarbon Fiber AmperometryDopamine Related GenesProteinsQuantitative PCRImmunocytochemistryRodent NeonatesCortical Glial AstrocytesConditioning Of Neuronal Cell Culture MediaMidbrain DissectionDigestionExtractionPlating Of Dopamine NeuronsNeurotrophic FactorsElectrophysiologyVideo MicroscopyCell Viability AssaysToxicological Screens
