29.12
Gap Junctions
Das Zytoplasma benachbarter tierischer Zellen kann über die Kommunikationskanäle, die die Gap Junctions bilden, kleine Moleküle, Ionen und sekundäre B…
Gap Junctions sind Kommunikationskanäle zwischen benachbarten tierischen Zellen.
Diese Kanäle bestehen aus Transmembranproteinen, den Connexinen. Sechs Connexin-Moleküle bilden einen Halbkanal, der als Connexon bezeichnet wird.
Ein homomeres Connexon besteht aus sechs Kopien desselben Connexin-Proteins, während ein heteromeres Connexon von verschiedenen Connexin-Proteinen gebildet wird.
Die Connexons können dynamisch durch die Membran diffundieren. Wenn ein Connexon auf einer Zellmembran auf ein anderes Connexon auf der benachbarten Zelle trifft, paaren sie sich zu einem vollständigen Kanal.
Cluster solcher Kanäle bilden die Gap-Junction-Plaques, die den Austausch von Ionen, sekundären Botenstoffen, Zuckern und anderen kleinen Molekülen zwischen Zellen ermöglichen.
Die Vielfalt der Connexine und ihrer Kombinationen verleiht der Selektivität der Kanäle für bestimmte Moleküle.
Der Transport von gelösten Stoffen wird weiter reguliert durch das Öffnen und Schließen von Kanälen als Reaktion auf verschiedene Reize, wie z. B. Spannungsdifferenz, Kalziumionenkonzentration und pH-Wert.
Zum Beispiel reagieren Gap Junctions im Herzmuskel auf Spannungsunterschiede und synchronisieren den Ionenfluss zwischen Gruppen von Muskelzellen, wodurch rhythmische Kontraktionen im Herzen erzeugt werden.
View the full transcript and gain access to JoVE Core videos
Q1: What are connexins and how do they form gap junction channels?
Connexins are transmembrane proteins that assemble into hexameric structures called connexons. Six connexin molecules form a hemichannel, which can be homomeric (identical connexins) or heteromeric (different connexins). When connexons from adjacent cells pair up, they create complete channels that form gap junction plaques, enabling molecular exchange between cells.
Q2: How do gap junctions regulate the transport of molecules between cells?
Gap junctions control molecular transport through channel selectivity determined by connexin diversity and combinations. Transport is further regulated by opening and closing channels in response to stimuli including voltage differences, calcium ion concentration, and pH. This dynamic regulation allows cells to selectively exchange ions, secondary messengers, sugars, and other small molecules based on cellular needs.
Q3: What is the difference between connexons and innexons in invertebrate gap junctions?
Invertebrate gap junctions use innexins (invertebrate connexins) instead of connexins. While innexins share a similar transmembrane structure, they lack sequence homology with vertebrate connexins. Crucially, innexons require eight units to form a hemichannel, whereas vertebrate connexons require only six units, reflecting structural differences between invertebrate and vertebrate communication channels.
Q4: How do connexons move and cluster to form gap junction plaques?
Connexons are synthesized on rough endoplasmic reticulum, transported through the Golgi network, and delivered to the plasma membrane via secretory vesicles. Once at the membrane, connexons freely diffuse until encountering other connexons, forming clusters. New connexons continuously add to cluster peripheries while old connexons at the center are endocytosed and degraded, creating dynamic, constantly remodeled gap junction plaques.
Q5: How do gap junctions in heart muscle synchronize cellular contractions?
Gap junctions in heart muscle respond to voltage differences across cell membranes. This voltage sensitivity allows rapid ion flow synchronization between adjacent muscle cells, coordinating their electrical activity. This synchronized ion exchange generates rhythmic contractions throughout the heart, enabling coordinated pumping action essential for effective circulation.
Q6: What structural differences exist between animal and plant cell-to-cell communication?
Animal cells use gap junctions where plasma membranes directly contact adjacent cells. Plant cells, separated by cell walls preventing direct membrane contact, instead use plasmodesmata—tubular channels 20-40 nanometers in diameter where one cell's membrane is continuous with the adjacent cell's membrane, allowing contiguous cytoplasm and regulated molecular exchange.
Q7: Why is connexin diversity important for gap junction function?
Connexin diversity enables channel selectivity for specific molecules through different protein combinations. Homomeric connexons use identical connexins, while heteromeric connexons combine different connexins, creating varied channel properties. This molecular diversity allows different cell types and tissues to establish specialized communication channels suited to their specific physiological requirements and signaling needs.