Sinoatrial Node Isolation from Mouse Model: A Procedure to Harvest SA Node from Murine Heart

Abstract

Source: Kumar, P et al., Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice. J. Vis. Exp. (2021)

This video demonstrates the procedure to isolate sinoatrial node or SAN from mouse heart. The SAN houses the sinoatrial cells that initiates heartbeat by generating spontaneous action potentials.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Dissecting the sinoatrial node (SAN)

1. Prepare heparinized Complete Tyrode's solution for SAN dissection.
   1. Add 400 µL of heparin (1,000 USP/mL) to 40 mL of Complete Tyrode's solution and warm in a 37 °C water bath.
2. Inject the mouse intraperitoneally with 200-300 μL of heparin (1000 USP/mL) and allow the animal to sit for 10 min.
3. Euthanize the heparinized mouse by isoflurane overdose.
   1. Place the mouse in a small glas....

Results

Figure 1: Removal of the heart. (A) Transverse incision in the skin just beneath the bottom of the rib cage from about the left costal arch to the righ.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
22 gauge syringe needle	Fisher Scientific	14-826-5A	Used for dissection
23 gauge syringe needle	Fisher Scientific	14-826-6C	Used for dissection
60mm Petri Dishes	Genesee Scientific	32-105G
Castroviejo Scissors, 4"	Fine Science Tools	15024-10
Dissection Microscope	Jenco
Dissecting Pins	Fine Science Tools	26002-20
Dumont #2 Laminectomy Forceps	Fine Science Tools	11223-20
Dumont #55 Forceps	Fine Science Tools	11295-51
 Extra Fine Graefe Forceps	Fine Science Tools	11152-10
Hemostat	Fine Science Tools	13013-14
Heparin	Aurobindo Pharma Limited IDA, Pashamylaram	NDC 63739-953-25
Sylgruard Elastomer Kit	Dow Corning	184 SIL ELAST KIT 0.5KG
Surgical Scissors	Fine Science Tools	14074-09
Transfer Pipets (3mL graduated)	Samco Scientific	225
Isoflurane 	Patterson Veterinary 	07-893-1389