Bodipy-c12
Bodipy-c12 - Related Videos
06:03
Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs
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2025
This protocol details an in vitro assay for measuring cellular lipid uptake in endothelial cells upon stimulation with BODIPY-C12 and BODIPY-C16 fluorescent analogs of long-chain and very long-chain saturated fatty acids. This method is efficient and adaptable to other cell types, offering a useful approach for studying lipid metabolism.
05:33
A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum
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2025
This video demonstrates Microneutralization assays to measure neutralizing antibodies using Madin-Darby Canine Kidney cells overexpressing α2,6-linked sialic acid in human sera. This assay is often used for influenza human...
10:58
Obtaining Specimens with Slowed, Accelerated and Reversed Aging in the Honey Bee Model
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Cited by 19 •
2013
In honey bee workers, aging depends on social behaviors rather than on chronological age. Here we show how worker-types with very different aging patterns can be obtained and analyzed for cellular senescence.
11:30
High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake
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Cited by 19 •
2016
Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.
12:47
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
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Cited by 8 •
2014
Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.
FAQs
The dodecanoic acid chain gives the probe lipid-like behavior, favoring association with membranes and participation in lipid-processing pathways. The attached BODIPY fluorophore supplies the detectable signal. Consequently, fluorescence measurements can connect where the analog is located with how lipid-associated processes distribute it through biochemical systems.
Changes in the probe’s location can indicate stages of cellular uptake, membrane incorporation, and trafficking between intracellular compartments. Following these patterns helps researchers examine how lipid-like molecules move through the cell rather than measuring membrane association as an isolated event. The resulting observations provide context for lipid transport and compartment-specific processing.
BODIPY-C12 links a chemically defined fatty-acid-like chain with an optical readout, allowing lipid behavior to be examined in biochemical systems. Its distribution can be related to membrane organization, lipid metabolism, and transport. These connections help researchers investigate how molecular lipid properties contribute to energy balance and metabolic regulation.
Researchers can examine BODIPY-C12 with fluorescence microscopy to resolve its distribution or with fluorescence spectroscopy to measure its signal in a system. Microscopy emphasizes localization and trafficking, whereas spectroscopy provides a fluorescence-based measurement without the same spatial emphasis. Selecting between them depends on whether the study prioritizes cellular position or overall signal.
The probe supports studies of fatty-acid transport, membrane dynamics, lipid metabolism, and interactions between lipid molecules and cellular compartments. It can therefore be applied when researchers need to relate lipid-like molecular behavior to cellular organization or processing. Its value is especially clear in experiments examining how membranes and metabolic pathways handle fatty-acid analogs.
In biochemistry, observations from BODIPY-C12 can be interpreted across two connected levels: membrane behavior and lipid processing. Membrane association reflects organization within lipid environments, while intracellular distribution relates to transport and metabolism. Together, these measurements help connect molecular lipid chemistry with broader questions about compartmentalization, energy balance, and metabolic control.