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Chapters
- 00:05Title
- 00:59Preparation of Fluorescent Lipids
- 03:03Preparation of Liposome Feed
- 04:15Feeding Paradigm and Mounting Larvae for Live Imaging
- 06:04Food Intake Assay
- 08:08Results: High-fat Feeding Fluorescent Analogs Allows for Visualization of Transport and Incorporation of Lipids
- 09:51Conclusion
Zebrafish are emerging as a valuable model of dietary lipid processing and metabolic disease. Described are protocols of lipid-rich larval feeds, live imaging of dietary fluorescent lipid analogs, and quantification of food intake. These techniques can be applied to a variety of screening, imaging, and hypothesis driven inquiry techniques.
