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Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
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Chapters
- 00:05Title
- 01:36Acute Hippocampal Slice Preparation
- 04:58Bolus Loading of Astrocytes with Ca2+ Indicator
- 07:22Recording Spontaneous and Gq G Protein-coupled Receptor Agonist-evoked Astrocytic Ca2+ Activity in Hippocampal Slices
- 09:59Results
- 11:48Conclusion
Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.
