3d Osteocyte Models
3d Osteocyte Models - Related Videos
07:13
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
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Cited by 19 •
2014
Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic...
09:18
A 3D Bone Culture Platform Using Human Osteocytes and Decellularized Extracellular Matrix for Modeling Musculoskeletal Diseases
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2026
This protocol describes the fabrication of 3D bone constructs using human osteocytes embedded in methacrylated gelatin (GelMA) hydrogels supplemented with decellularized extracellular matrix. The method is low-cost, scalable, and compatible with basic laboratory equipment, providing a translational platform for studying musculoskeletal disease processes.
10:19
Analysis and Imaging of Osteocytes
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Cited by 1 •
2024
This study outlines the method to visualize and develop three-dimensional (3D) models of osteocytes within the lacunar-canalicular network (LCN) for computational fluid dynamics (CFD) analysis. The generated models using this method help to understand osteocyte mechanosensation in healthy or diseased bones.
09:17
3D Printing of Biomolecular Models for Research and Pedagogy
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Cited by 29 •
2017
Physical models of biomolecules can facilitate an understanding of their structure-function for the researcher, aid in communication between researchers, and serve as an educational tool in pedagogical endeavors. Here, we provide detailed guidance for the 3D printing of accurate models of biomolecules using fused filament fabrication desktop 3D printers.
07:22
Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes
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Cited by 4 •
2020
This protocol describes the dissection of neonatal dmp1-topaz mouse calvaria and isolation of osteocytes expressing the green fluorescent protein through cell digestion and fractionation, in addition to osteocyte preparation for fluorescence activated cell sorting (FACS).
FAQs
The surrounding matrix provides the physical context for osteocyte-matrix interactions, which are central to how these cells develop and function. Mineralized matrices, hydrogels, and porous scaffolds can each serve as engineered environments for embedding osteocytes or osteocyte-like cells. Selecting one of these formats helps researchers recreate three-dimensional conditions relevant to bone remodeling and mechanosensory function.
Mechanical loading and fluid flow provide controlled stimuli that allow researchers to examine how osteocytes respond to physical conditions. These inputs support studies of mechanotransduction, the process by which mechanical signals influence cellular behavior. Applying them within a three-dimensional matrix or scaffold makes it possible to investigate responses in an environment designed to resemble the osteocyte’s functional setting.
Three-dimensional osteocyte models represent cell-matrix interactions more closely than conventional two-dimensional cultures. Their embedded or scaffold-based format provides a spatial environment in which osteocytes or osteocyte-like cells can experience engineered three-dimensional surroundings and controlled physical stimuli. This difference makes them useful for studying cellular responses that may be difficult to represent in flat culture systems.
The principal design components are the osteocyte or osteocyte-like cell population, the surrounding mineralized matrix, hydrogel, or porous scaffold, and the applied mechanical loading or fluid flow. Together, these elements establish the cellular, structural, and stimulus conditions of the model. Changing this combination allows bioengineers to examine different aspects of bone-cell function and mechanotransduction.
A basic workflow begins by selecting osteocytes or osteocyte-like cells and placing them within a mineralized matrix, hydrogel, or porous scaffold. The engineered construct is then exposed to controlled mechanical loading or fluid flow when the experiment requires physical stimulation. Researchers can use the resulting system to study cell-matrix interactions, mechanotransduction, or responses relevant to bone biology.
Researchers can use 3D osteocyte models when they need a laboratory platform for investigating skeletal disease, evaluating biomaterials, or examining therapeutics. Because the systems reproduce relevant three-dimensional cellular and mechanical conditions, they support studies of how engineered materials or treatment strategies relate to osteocyte behavior. The same platforms can also inform approaches to bone regeneration.
These models provide a setting for investigating osteocyte communication with other bone cells while maintaining a three-dimensional environment. That capability connects osteocyte mechanosensory behavior with broader questions about bone remodeling. In bioengineering, such systems can therefore help relate cellular responses to mechanical stimuli and matrix conditions to interactions that are relevant to engineered bone and regenerative research.