Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

JoVE Journal Biology
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
 

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Article doi: 10.3791/51465
May 13th, 2014 •
1, 2, 1, 1, 1, 3, 1, 1, 1, 1
1Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 2Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 3Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

Summary
Automatic Translation
May 13th, 2014

Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic models. 

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter