Antimicrobial Surface Modification
Antimicrobial Surface Modification - Related Videos
12:31
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Cited by 14 •
2012
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
14:48
PDMS Device Fabrication and Surface Modification
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Cited by 5 •
2007
11:52
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
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Cited by 1 •
2023
We introduce four methods to evaluate the antimicrobial activities of nanoparticles and nanostructured surfaces using in vitro techniques. These methods can be adapted to study the interactions of different nanoparticles and nanostructured surfaces with a broad range of microbial species.
04:12
An In Vitro Technique to Study Antimicrobial Treatments on Bacterial Aggregates
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2025
This video demonstrates an in vitro technique to study the impact of antimicrobial treatment on Pseudomonas aeruginosa (Pa) aggregates. Fluorescently-labeled Pa cells are grown as aggregates, mimicking the growth pattern during infection. Upon challenging the aggregates with a sublethal concentration of the antibiotic colistin, the real-time assessment of the bacteria-antibiotic interaction is performed via fluorescence microscopy.
11:10
Expression, Purification, and Antimicrobial Activity of S100A12
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Cited by 14 •
2017
Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.
FAQs
These approaches act at different stages of microbial colonization. Contact-killing surfaces act when microorganisms touch the modified layer, whereas controlled-release designs deliver antimicrobial agents from the surface over time. Biofilm-preventing strategies focus on limiting the development of organized microbial communities. Selecting among them depends on the intended duration and mode of protection for the bioengineering device.
A surface can reduce microbial survival yet remain unsuitable if it harms host cells or loses structural integrity. Antimicrobial surface modification therefore requires simultaneous attention to low toxicity, compatibility with host cells, and mechanical stability. This balance is especially important for implanted or tissue-contacting devices, where antimicrobial performance must support rather than compromise the device’s biological function.
The outer layer of a material affects whether microorganisms attach, persist, or grow, while also influencing how the material interacts with surrounding biological tissues. In bioengineering, modifying that layer can reduce opportunities for colonization without changing the entire device. The resulting surface must retain useful mechanical behavior and remain compatible with host cells during use.
Three supported routes are coatings, chemical grafting, and incorporation of antimicrobial agents. A coating adds an engineered outer layer, chemical grafting modifies the surface through attached chemical groups, and incorporation places antimicrobial components within the material system. The selected route should match the intended contact-killing, controlled-release, or biofilm-prevention function and the required mechanical stability.
Applications include implants, catheters, wound dressings, tissue-engineering scaffolds, and diagnostic devices. These platforms differ in their contact with tissues, fluids, or external environments, but each can be affected by microbial attachment and growth. Surface modification provides a way to address contamination or device-associated infection while preserving the broader requirements of biocompatibility and functional performance.
Assessment should consider whether the modified surface reduces microbial attachment, survival, or growth, and whether that activity remains durable. Researchers must also examine toxicity, mechanical stability, and compatibility with host cells. Considering these outcomes together helps distinguish a surface that is merely antimicrobial from one that is suitable for practical bioengineering applications such as implants or scaffolds.