Bhi Urea Medium
Bhi Urea Medium - Related Videos
08:44
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
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Cited by 104 •
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Denaturing urea polyacrylamide gel electrophoresis is used to separate single-stranded DNA or RNA up to a limit of 500 nucleotides. Urea in combination with heat denatures samples and unstructured single strands migrate within the gel matrix according to their molecular weight.
01:23
Urea Cycle
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2025
The urea cycle describes how liver cells convert ammonia to urea. Ammonia is a toxic waste product of protein catabolism. Land animals must convert ammonia into the less toxic urea which can be safely eliminated by the kidneys through urine. Marine animals excrete ammonia directly, and the surrounding water dilutes the ammonia to safe levels. There are five basic steps in the urea cycle: the conversion of ammonia (NH3) to carbamoyl phosphate the introduction of ornithine in the...
07:52
Removal of Branched and Cyclic Compounds by Urea Adduction for Uk'37 Paleothermometry
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst As mentioned in previous videos, the product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. In the case of our two organic paleothermometers (Uk'37 and MBT/CBT), the interest is in only 6 compounds (2 alkenones and 4 isoprenoidal glycerol dialkyl glycerol...
03:01
Organ Conditioned Medium: Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies
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2023
This video describes a method of generating an organ conditioned medium derived from the mouse liver. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.
13:33
Maintaining Wolbachia in Cell-free Medium
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Cited by 6 •
2007
This video describes a method for purifying Wolbachia pipientis from an Anopheles gambiae cell line and then culturing the endosymbiont in cell-free medium. An assay for viability of the bacterium is demonstrated.
FAQs
Urease hydrolyzes the supplied urea, generating ammonia that raises the medium’s pH. Phenol red responds to this alkalinization by shifting the medium from yellow toward pink or red. The color change therefore links a visible chemical response to enzymatic substrate breakdown, allowing investigators to recognize urease activity in a cultured microorganism.
The two major components support different parts of the test. Brain heart infusion provides peptides and other growth factors needed to support microbial growth, while urea supplies the substrate whose breakdown reveals urease activity. Separating these functions helps the medium both sustain the culture and generate a biochemical signal for characterization.
The pH response converts an enzymatic reaction into an interpretable color outcome. Ammonia accumulation indicates that urea hydrolysis has occurred, and the associated shift toward pink or red supports classification as urease-producing. Because identification can involve multiple traits, this result is most informative when considered with other observations rather than treated as the sole characterization method.
A typical workflow begins with introducing the cultured microorganism into BHI Urea Medium, followed by observing the medium for the indicator response associated with urease activity. A yellow medium indicates no described alkalinization signal, whereas development of pink or red supports urea hydrolysis. The observed outcome can then be recorded for biochemical identification.
In bioengineering, the medium is useful when researchers need to screen strains for urease production or characterize microbial metabolism. It can contribute to diagnostic workflows and help distinguish organisms with different biochemical properties. Because the assay produces a visually interpretable result, it provides a practical screening step before broader characterization or comparative strain analysis.
The assay provides biochemical evidence that can complement colony morphology, microscopy, and additional biochemical tests. For example, a color response indicating urease activity may be evaluated alongside visible colony features and microscopic observations to strengthen microbial characterization. Combining these data helps researchers distinguish related isolates and build a more complete profile of a cultured strain.