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JoVE Journal
Biology

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Maintaining Wolbachia in Cell-free Medium
 

Maintaining Wolbachia in Cell-free Medium

Article DOI: 10.3791/223
July 4th, 2007

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Summary July 4th, 2007

This video describes a method for purifying Wolbachia pipientis from an Anopheles gambiae cell line and then culturing the endosymbiont in cell-free medium. An assay for viability of the bacterium is demonstrated.

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