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Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
 

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

Article DOI: 10.3791/1485
October 29th, 2009

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Summary October 29th, 2009

Denaturing urea polyacrylamide gel electrophoresis is used to separate single-stranded DNA or RNA up to a limit of 500 nucleotides. Urea in combination with heat denatures samples and unstructured single strands migrate within the gel matrix according to their molecular weight.

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