Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

In general, this type of gel is required to separate single stranded DNA or RNA fragments such as synthesized or labeled oligonucleotides or products from enzymatic cleavage reactions. The separation range is dependent on the polyamide concentration, uria in combination with heat denas to sample and single stranded molecules migrate within the gel matrix according to their molecule weight. This video presents the pouring, running, and processing of a typical gel.

Hello, my name is Haki. I'm with Nang Technological University. In this demonstration, I'm going to show you how to perform A Denaturing poly gel electrophoresis.