Bioanalyzer
Bioanalyzer - Related Videos
08:37
Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer
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2024
Alcohol misuse impairs alveolar macrophage (AM) immunity due to suppressed mitochondrial respiration and bioenergetics. We recently demonstrated that ethanol (EtOH) exposure increases glutamine dependency for mitochondrial respiration in AMs. Herein, methods are provided to determine the usage of glutamine for mitochondrial respiration in EtOH-treated AMs using an extracellular flux bioanalyzer.
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Cited by 9 •
2016
Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.
02:43
Assessing Mitochondrial Function in Cerebral Vascular Endothelial Cells
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2025
This video demonstrates the assessment of the mitochondrial functioning of cerebral vascular endothelial (CVE) cells using an extracellular flux assay. CVE cells are plated in an extracellular flux cell culture plate. Extracellular flux sensors are hydrated before loading and calibration into the bioanalyzer. The plate containing cells is then loaded for analysis.
09:15
DNA-based Fish Species Identification Protocol
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Cited by 9 •
2010
This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.
09:27
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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Cited by 10 •
2011
We demonstrate the use of DNA microarrays for expression profiling of the nervous system. We describe RNA quality control, sample labeling, and array hybridization and scanning.
FAQs
The system separates DNA, RNA, or protein molecules by electrophoresis inside a disposable lab-on-a-chip format. Differences in molecular size determine how fragments move through the microfluidic separation system. Fluorescent detection then records the separated material, producing an electropherogram that displays fragment patterns and supports assessment of concentration, size, and integrity.
Fluorescent detection converts separated biomolecule fragments into measurable signals that can be displayed in an electropherogram. This output helps researchers assess fragment size and concentration while also identifying patterns associated with degraded or contaminated samples. The resulting measurements provide a more informative quality assessment than relying only on whether a sample is present.
Disposable lab-on-a-chip systems integrate microfluidic separation into a compact analysis format, allowing biomolecules to be evaluated within the Bioanalyzer workflow. Their role is especially relevant because separation and detection occur in the same instrument-based process. This supports rapid quality control when researchers need to screen samples before continuing with larger molecular experiments.
Electropherograms provide a visual and quantitative profile of separated fragments, allowing researchers to recognize degradation, contamination, or insufficient sample quality. These findings can guide decisions about whether a sample is suitable for sequencing, polymerase chain reaction, or gene expression studies. Detecting problems before those workflows helps reduce the risk of unreliable downstream results.
Researchers analyze the relevant DNA, RNA, or protein sample with the instrument and examine the resulting electropherogram and quality metrics. They then use measurements of size, concentration, and integrity to decide whether the material is appropriate for the planned workflow. This quality-control step is performed before sequencing, polymerase chain reaction, gene expression studies, or related molecular work.
The analysis is most useful before a downstream experiment depends on reliable biomolecule quality. Researchers can use it to screen samples before sequencing, polymerase chain reaction, gene expression studies, and other molecular workflows. Early assessment identifies problematic material before it influences later results, helping researchers make informed decisions about sample suitability and experimental reliability.
Within bioengineering, Bioanalyzer analysis supports quality control for studies involving nucleic acids and proteins. Its results can inform work in diagnostics, synthetic biology, and biomaterials research by showing whether samples have suitable size, concentration, and integrity. The technique therefore connects instrument-based molecular assessment with decisions about experimental readiness and interpretation.