Bioprocess Monitoring
Bioprocess Monitoring - Related Videos
07:42
Overview of Bioprocess Engineering
0 Views •
2023
Bioprocessing is a method that uses living organisms to produce a desired target product. Often, bioprocessing refers to the use of bioreactors to produce protein products from genetically engineered organisms. This field is responsible for the large-scale manufacture of biotherapeutics; drugs that have become essential to improving the quality of life for many with complex diseases like cancer, autoimmune diseases and HIV/AIDS. This video will introduce the engineering approach to designing a...
07:26
In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
0 Views •
Cited by 5 •
2019
A photo-optical in situ microscopy device was developed to monitor the size of single cells directly in the cell suspension. The real-time measurement is conducted by coupling the photo-optical sterilizable probe to an automated image analysis. Morphological changes appear with dependence on the growth state and cultivation conditions.
08:43
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
0 Views •
Cited by 29 •
2017
Proton Transfer Reaction Time of Flight Mass Spectrometry allows high-sensitivity, rapid and non-invasive analysis of volatile organic compounds. To demonstrate its potential, we give three examples: lactic acid fermentation of yogurt (on-line bioprocess monitoring), different apple genotypes (large-scale screening), and retronasal space after drinking coffee (nosespace analysis).
02:05
Self-Presentation: Self-Monitoring and Self-Handicapping
0 Views •
2020
People can go to great lengths to protect their self-image and present themselves in ways that they want others to see them. Sociologist Erving Goffman presented the idea that a person is like an actor on a stage. Calling his theory dramaturgy, Goffman believed that we use “impression management” to present ourselves to others as we hope to be perceived. Each situation is a new scene, and individuals perform different roles depending on who is present (Goffman, 1959). Think about the way you...
01:23
Holter Monitor: 24-Hour Monitoring
0 Views •
2025
Holter monitoring is a continuous electrocardiography (ECG) recording that tracks the heart's electrical activity over an extended period, generally 24 to 48 hours. This noninvasive diagnostic tool detects irregular heart rhythms that may not be captured during a standard ECG performed in a clinical setting.DeviceThe Holter monitor is a portable, small device connected to several electrodes on the patient's chest. These electrodes detect the heart's electrical signals and transmit them to the...
FAQs
Its value comes from interpreting measurements in relation to process performance and desired product quality. Changes in temperature, pH, dissolved oxygen, biomass, nutrients, or metabolites can indicate that conditions are shifting. Teams can then use the information to adjust process conditions, helping maintain consistency, identify deviations, and support more reliable production outcomes.
Online measurements are obtained during the process, while at-line measurements are performed near the production system, and offline measurements are conducted through separate analysis. These approaches provide different ways to assess process variables and interpret changes. Selecting among them helps teams track biological production conditions and obtain information needed for timely adjustments or later evaluation.
A single measurement may not fully describe the state of a biological production process. Temperature and pH show environmental conditions, dissolved oxygen reflects another important process condition, and biomass, nutrients, and metabolites provide information about growth and production. Considering these measurements together supports a more complete interpretation of performance and helps reveal deviations that could affect yield or quality.
Both systems require assessment of process conditions and biological performance, but monitoring must reflect the production system being used. Measurements of temperature, pH, dissolved oxygen, biomass, nutrients, and metabolites can inform adjustments in either microbial fermentation or mammalian cell culture. Applying the same monitoring principle to each system supports consistent operation while accounting for their distinct biological contexts.
A practical workflow begins by selecting relevant process variables, measuring them through online, at-line, or offline approaches, and interpreting the resulting data. Teams then compare the observed conditions with expected process performance, identify deviations, and adjust conditions when appropriate. Repeating this cycle supports process control, improves reproducibility, and provides information for evaluating production outcomes.
Monitoring becomes particularly important when a process moves from laboratory systems toward manufacturing, because teams must determine whether performance remains consistent across scales. Tracking conditions and biological responses can reveal deviations, support process control strategies, and inform efforts to maintain yield and product quality. This context is relevant to producing vaccines, therapeutics, and other bio-based products.