Bioreactor Vessel

A bioreactor vessel is a controlled container used to support biological reactions by providing conditions that enable cells, enzymes, or microorganisms to grow or produce a desired product. Within the vessel, mixing promotes uniform distribution of nutrients and gases, while monitoring and regulating variables such as temperature, pH, dissolved oxygen, and agitation helps maintain a suitable environment for the biological system. In bioengineering, bioreactor vessels are used to cultivate cells, produce biologics, develop tissues, and study engineered biological processes. Their controlled operation supports reproducible experiments, scalable manufacturing, and the optimization of products and production systems.

Bioreactor Vessel - Related Videos

Research

JoVE Journal - Biology

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

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Cited by 80 •

2012

A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.

Research

JoVE Journal - Bioengineering
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Operation of a Benchtop Bioreactor

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Cited by 9 •

2013

Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.

Education

JoVE Science Education - Engineering

Batch and Continuous Bioreactors

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2023

Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.

Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor

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Cited by 1 •

2023

This protocol presents the setup and operation of a newly developed, 3D printed bioreactor for the ex vivo culture of blood vessels in perfusion. The system is designed to be easily adopted by other users, practical, affordable, and adaptable to different experimental applications, such as basic biology and pharmacological studies.

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

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Cited by 32 •

2011

Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.

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