Electron Spin Resonance
Electron Spin Resonance - Related Videos
09:40
Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
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Cited by 3 •
2010
This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.
11:07
Electron Paramagnetic Resonance (EPR) Spectroscopy
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2023
Source: David C. Powers, Tamara M. Powers, Texas A&M In this video, we will learn the basic principles behind Electron Paramagnetic Resonance (EPR). We will use EPR spectroscopy to study how dibutylhydroxy toluene (BHT) behaves as an antioxidant in the autoxidation of aliphatic aldehydes.
13:21
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
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Cited by 40 •
2012
Electron paramagnetic resonance (EPR) spectroscopy was employed to detect nitric oxide from bovine aortic endothelial cells and superoxide radical anion from human neutrophils using iron (II)-N-methyl-D-glucamine dithiocarbamate, Fe(MGD)2 and 5,5-dimethyl-1-pyroroline-N-oxide, DMPO, respectively.
07:33
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
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Cited by 5 •
2010
This technique enables the use of high-resolution magic angle spinning proton MR spectroscopy (HRMAS 1H-MRS) for molecular characterization of live Drosophila melanogaster with a conventional 14.1 tesla spectrometer equipped with an HRMAS probe.
08:01
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
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Cited by 11 •
2016
A new electron paramagnetic resonance (EPR) method, rapid scan EPR (RS-EPR), is demonstrated for 2D spectral spatial imaging which is superior to the traditional continuous wave (CW) technique and opens new venues for in vivo imaging. Results are demonstrated at 250 MHz, but the technique is applicable at any frequency.
FAQs
The resonance condition depends on matching the energy of a microwave photon to the separation between electron spin energy levels in an applied magnetic field. When the energies coincide, the sample absorbs microwave radiation, producing a measurable signal. This relationship allows ESR spectra to report how paramagnetic species respond to their magnetic and chemical environments.
The g-factor and hyperfine interactions provide complementary structural information in an ESR spectrum. The g-factor reflects characteristics of the electron spin response, while hyperfine interactions reveal coupling between the unpaired electron and nearby magnetic nuclei or centers. Examining these parameters helps researchers characterize the structure and chemical environment of paramagnetic molecules or materials.
Unpaired electrons create electron spin states that can separate in an applied magnetic field and undergo microwave-driven transitions. Species without the relevant unpaired electrons do not provide the same ESR response. This selectivity makes the technique particularly useful for examining free radicals and paramagnetic metal centers within complex biological samples or engineered materials.
A sample is placed in an applied magnetic field while exposed to microwave radiation. The measurement records absorption when the microwave photon energy matches an electron spin transition. Researchers then examine the resulting spectrum, including its g-factor and hyperfine features, to characterize the sample’s paramagnetic components and their chemical environments.
ESR can characterize free radicals, which are relevant to oxidative stress and cellular redox processes. Detecting and examining these paramagnetic species provides information about chemical changes associated with redox activity. In bioengineering research, that information can contribute to studies of cellular behavior, tissue damage, and systems designed to monitor or influence these processes.
Bioengineers apply ESR when they need information about paramagnetic metal centers, free radicals, or other spin-active features in biological and engineered systems. The technique supports investigations of enzyme mechanisms, tissue damage, and biomaterial behavior. It also contributes to designing diagnostic or therapeutic systems by characterizing relevant chemical states in those applications.