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Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Article DOI: 10.3791/2122 • 09:40 min • August 26th, 2010
August 26th, 2010 •
1, 1, 1, 1
1Schulich Faculty of Chemistry, The Technion, Israel Institute of Technology

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Summary
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This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.

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Electron Spin Resonance ESR Micro-imaging Oxygen Mapping Live Cells Oxygen Levels Mitochondrial Functions Metabolic Functions Signaling Pathways Membrane Permeability Disease Differentiation Cellular Metabolism High-resolution Oxygen Imaging Optical Fluorescence Phosphorescence Methods Exogenous Paramagnetic Probes
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