Macrophage Cell Assay
Macrophage Cell Assay - Related Videos
03:00
An Assay to Study the Regulation of Macrophage Phagocytosis by Mesenchymal Stem Cells
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2025
This video demonstrates an assay to study mesenchymal stem cell (MSC) regulation of macrophage phagocytosis in a co-culture. Upon co-culturing MSC with macrophages, non-opsonized zymosan particles conjugated to a pH-sensitive fluorophore are added to be phagocytosed by the macrophages. With the increase in phagocytosis, the fluorescence intensity rises, allowing the determination of MSC-mediated regulation of phagocytic activity.
04:57
Fixed Cell Efferocytosis Assay: A Method to Study Efferocytic Uptake of Apoptotic Cells by Macrophages Using Fluorescence Microscopy
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2025
In this video, we demonstrate the efferocytosis assay to study the clearance of apoptotic cells using confocal microscopy. Efferocytosis is a multi-step process where apoptotic cells are engulfed by phagocytes and degraded within vesicles called efferosomes. This protocol provides a method to visualize internalized and non-internalized portions of individual apoptotic cells and quantify efferocytosis.
06:23
Microglial Phagocytosis Assay: An In Vitro Assay to Establish Microglial Phagocytosis Model for Neuroblastoma Cells Using iPSC Macrophages
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2023
In this video, we perform the microglial phagocytosis assay for neuroblastoma using induced pluripotent stem cells. The assay helps assess the impairment of microglial function in neurodegenerative diseases.
09:23
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Cited by 19 •
2016
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
03:29
An In Vitro Assay to Quantify the Intracellular Growth of Parasite in Macrophages
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2025
This video demonstrates an assay to quantify the intracellular growth of parasites in bone marrow-derived macrophages (BMMs). The macrophage cells are infected with the motile and non-motile invasive stages of Leishmania, metacyclic promastigotes and amastigotes, respectively. The parasites invade the macrophages, getting internalized into a parasitophorous vacuole and multiplying inside. The intracellular parasite growth is quantified using a microscope upon fluorescently staining the...
FAQs
It links a defined exposure to measurable macrophage outcomes. Phagocytosis indicates particle or target uptake, cytokine secretion reflects signaling activity, viability shows whether the condition is harmful, and surface markers provide evidence of cellular state. Examining these readouts together helps distinguish general toxicity from changes in immune activation or phenotype.
Each readout addresses a different aspect of the response. Phagocytosis measures uptake behavior, cytokines indicate secreted inflammatory signaling, viability identifies effects on cell survival, and surface markers characterize changes associated with macrophage activation and polarization. Selecting complementary measurements gives a broader interpretation than relying on a single indicator.
Macrophage state can influence how engineered materials and tissue environments interact with the host. Measuring activation and polarization helps investigators determine whether a candidate material, delivery system, implant, or therapeutic environment is associated with an intended immune response. This information supports designs that seek to regulate inflammation and improve integration with host tissue.
Cultured macrophages are first maintained as the experimental cell system, then exposed to a defined biological stimulus, material, or therapeutic candidate. The resulting response is evaluated with selected measurements such as phagocytosis, cytokine secretion, viability, or surface markers. Keeping the exposure defined makes responses easier to compare across candidate conditions and engineering designs.
The assay can be applied to biomaterials, drug-delivery systems, implants, and engineered tissue environments. Investigators examine whether these candidates alter macrophage behavior, inflammatory signaling, survival, or phenotype. Such testing provides an immune-response perspective during material optimization and helps identify designs that may be more compatible with intended host-tissue interactions.
Results can guide material optimization, biocompatibility testing, and development of therapies designed to direct immune responses. A candidate may be compared according to its effects on uptake, cytokine release, viability, or surface markers, while phenotype-related findings add context about macrophage behavior. Together, these outcomes help bioengineers refine implants, delivery platforms, and tissue-engineering environments.