Silicon Probe Preparation
Silicon Probe Preparation - Related Videos
17:37
Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents
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Cited by 111 •
2012
We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication.
05:45
Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat
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Cited by 24 •
2011
Extracellular recordings of neuronal activity using silicon probes in the anesthetized rat will be described. This technique allows information to be obtained across multiple brain areas from more than 100 neurons simultaneously. It provides information with single cell resolution about neuronal ensembles dynamics in multiple local circuits.
11:25
Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
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Cited by 4 •
2016
We describe key steps for biosensing by using polysilicon nanowire field-effect transistors, including the preparation of the device and the immobilization and confirmation of a DNA molecular probe on the nanowire surface, as well as conditions for DNA sensing.
03:22
Recording Brain Electrical Activity in Behaving Mice Using Multi-Shank Linear Silicon Probes
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2025
This video outlines a protocol for implanting a multi-shank linear silicon probe into the mouse brain to measure local field potentials. The procedure involves securing the probe to a stereotaxic unit, positioning it accurately over the target brain region, inserting it into the brain, and securing it with dental cement. After implantation, the assembly is protected with a casing. Following recovery, the setup allows for recording local field potentials, providing insights into neuronal...
12:03
Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
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Cited by 9 •
2015
Reproducible cleaning processes for substrates used in DNA origami research are described, including bench-top RCA cleaning and derivatization of silicon oxide. Protocols for surface preparation, DNA origami deposition, drying parameters, and simple experimental set-ups are illustrated.
FAQs
The probe’s geometry determines how its electrode sites interface with cells or tissues and how it connects to recording hardware. Preparation therefore has to expose or connect the intended sites without deforming or damaging the microfabricated structure. Preserving this geometry supports consistent bioelectrical measurements, improves reproducibility between experiments, and helps maintain compatibility with biological interfaces.
Electrode sites must remain accessible for measuring or delivering bioelectrical signals, while other probe regions require electrical insulation. Preparation establishes this contrast by exposing or connecting selected sites and insulating the surrounding structure. The resulting configuration directs electrical interaction toward the intended locations, helping researchers obtain interpretable signals and maintain compatibility with recording or stimulation hardware.
Contamination can compromise the probe’s interaction with biological samples and reduce confidence in experimental results, while physical damage may alter the microfabricated geometry, electrode access, insulation, or hardware connection. Careful cleaning and handling limit these risks during preparation. Consistent control of both factors contributes to more reliable signal quality, repeatable experiments, and stable use in biological measurements.
Preparation combines fabrication, cleaning, assembly, and handling rather than relying on a single treatment. These stages ready the silicon structure, establish electrode exposure and insulation, and connect the probe to recording hardware. Their order and execution must protect the microfabricated geometry while limiting contamination and damage, leaving the device suitable for subsequent measurements or biological interfacing.
Handling affects whether the prepared probe reaches the experiment with its geometry, electrode sites, insulation, and hardware connections intact. Gentle, controlled handling helps prevent physical damage and preserves the configuration established during fabrication, cleaning, and assembly. This is important because intact preparation supports reliable bioelectrical measurements and improves consistency across biological experiments involving cells or tissues.
Prepared probes support measurements of bioelectrical signals from cells or tissues, making them relevant to neural activity studies and evaluations of engineered biological interfaces. They also contribute to development work involving diagnostics, stimulation, and physiological monitoring. In each case, consistent preparation helps connect the microfabricated device to biological experiments while supporting signal quality, reproducibility, and hardware compatibility.