Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Michelle A. Pillers; Rebecca Shute; Adam Farchone; Keenan P. Linder; Rose Doerfler; Corey Gavin; Valerie Goss; Marya Lieberman

doi:10.3791/52972

Journal

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Chemistry

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Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

14,259 Views

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12:03 min

•

July 23, 2015

DOI:

10.3791/52972-v

DOI:

10.3791/52972-v

•

12:03 min

July 23, 2015

•

1 Views

Michelle A. Pillers¹, Rebecca Shute¹, Adam Farchone², Keenan P. Linder³, Rose Doerfler², Corey Gavin⁴, Valerie Goss³, Marya Lieberman¹

¹Department of Chemistry and Biochemistry,University of Notre Dame, ²Department of Chemical and Biomolecular Engineering,University of Notre Dame, ³Department of Chemistry, Physics, and Engineering Studies,Chicago State University, ⁴Department of Technology,Ivy Tech Community College, South Bend, Indiana

Summary

Reproducible cleaning processes for substrates used in DNA origami research are described, including bench-top RCA cleaning and derivatization of silicon oxide. Protocols for surface preparation, DNA origami deposition, drying parameters, and simple experimental set-ups are illustrated.