Bifunctional Chimeric Protein
Bifunctional Chimeric Protein - Related Videos
04:43
β-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions
0 Views •
2025
This video demonstrates a conductimetric biosensor assay to study the interaction of an antibody with a target antigen immobilized on a transducer chip. The biosensor is engineered to have a bifunctional chimeric protein — a β-lactamase enzyme with a functional catalytic site fused to a nanobody for binding to the immobilized target antigen. Upon binding, the catalytic hydrolysis of an antibiotic substrate by the β-lactamase enzyme generates an electrical signal — confirming the...
10:33
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Cited by 3 •
2014
Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.
11:39
Identification of Functional Protein Regions Through Chimeric Protein Construction
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2019
Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence.
09:45
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
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2018
We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. It takes advantage of using a single expression plasmid that contains multiple functionally independent protein expressing cassettes to achieve protein co-expression.
21:01
Generating Chimeric Zebrafish Embryos by Transplantation
0 Views •
Cited by 61 •
2009
A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.
FAQs
Its two engineered domains can perform complementary tasks within the same molecular construct. One domain may recognize or bind a selected target, while the other carries out an associated activity, such as catalysis, signaling regulation, or cellular localization. Linking these functions can connect target recognition directly to the intended molecular response.
The usefulness of the construct depends on preserving the complementary behavior of both incorporated sequences. If the recognition domain no longer binds its target or the second domain loses its activity, the intended coordination cannot occur. Construct design therefore focuses on combining sequences without eliminating the functional roles that make the fusion informative or useful.
A single fused construct places recognition and action in one molecular unit rather than distributing them between separate proteins. This arrangement can simplify the conceptual connection between a target and its response, support coordinated activity, and help researchers study how linked functions influence molecular interactions, signaling, localization, or delivery.
Researchers first select two protein sequences with complementary functions, then genetically fuse their coding sequences into one construct. The resulting design is examined in the context of the intended task, such as binding, catalysis, signaling regulation, or localization, to determine whether both activities remain useful together.
They are useful when an experiment benefits from connecting a molecular target to a defined action. Supported applications include targeted delivery, molecular detection, pathway manipulation, and therapeutic design. The approach is especially relevant when researchers want one engineered molecule to link recognition with a biological response or to improve selectivity.
By combining distinct functional sequences, researchers can create a molecular tool that links target recognition with signaling regulation or another measurable biological action. Such constructs help investigate protein interactions, influence pathway behavior, and examine how molecular localization or binding relates to downstream biological effects within a coordinated design.