Exogenous Dna Delivery
Exogenous Dna Delivery - Related Videos
03:48
Hydrodynamic Gene Delivery: A Technique for In Vivo Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection
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2025
This video describes the technique of genetic in vivo transfection of murine hepatocytes by hydrodynamic tail vein injection. This transfection system generates a long-term expression of the transgene in the hepatocytes.
06:37
Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
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Cited by 6 •
2012
Skin tattooing is a potent and safe way to delivery DNA vaccine intradermally. Here, a DNA plasmid encoding EGFP is delivered by tattooing to the skin of a laboratory mouse, and the expression of EGFP in the skin cells is then inspected by confocal microscopy.
03:12
Mosquito Embryo Microinjection: A Technique to Deliver Exogenous DNA into Embryo Yolk of Anopheles Gambiae to Generate Germline Mutations
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2025
In this video, we demonstrate the delivery of gene drive construct into the embryo of Anopheles gambiae mosquito through embryo microinjection technique. This procedure allows for integration of delivered DNA construct, through homologous recombination, into the germline cells to generate transformed mosquito population.
09:42
Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
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2017
This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.
05:30
Exogenous Mitochondrial Transfer in Differentiating Brown Adipocytes and AGPAT2-Deficient Preadipocytes
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2026
Exogenous mitochondria of human and murine origin can be internalized by differentiating preadipocytes and tracked throughout adipogenic differentiation using imaging and genetic tools. While these mitochondria physically interact with the endogenous mitochondrial network, they do not increase the expression of markers associated with mature brown adipocytes.
FAQs
The outcome depends on more than DNA entering a cell. The delivery system must help DNA cross the plasma membrane and reach the nucleus; afterward, cellular conditions determine whether it is expressed or maintained. Consequently, a successful experiment must consider both intracellular access and the intended outcome, rather than treating uptake alone as evidence of functional delivery.
Chemical carriers, physical electroporation, and viral vectors represent alternative routes for introducing DNA. The choice should be matched to the desired balance of delivery efficiency, cell viability, DNA dosage, and expression duration. Because these variables can favor different systems, method selection is an optimization decision tied to the experiment, not a universally superior technique.
Transient transfection emphasizes temporary gene expression, whereas stable genetic modification aims to retain introduced genetic information. This distinction matters because expression duration is one of the key criteria for evaluating a delivery system. A short-term study may prioritize transient output, while longer-term experiments require maintenance of the modification under suitable cellular conditions.
Optimization should track delivery efficiency, cell viability, DNA dosage, and expression duration together. Increasing one outcome is not automatically beneficial if it compromises another, particularly cell viability. A practical optimization plan therefore compares conditions using both how effectively DNA is delivered and what happens to cells and expression afterward, allowing the system to be selected for the study’s specific goal.
A supported workflow begins by defining the intended outcome, such as transient expression, stable modification, recombinant protein production, or functional analysis. Researchers then select a chemical, physical, or viral approach and introduce DNA under conditions appropriate to the cells or tissue. Evaluation should include delivery efficiency, viability, and expression or maintenance.
Its value extends beyond changing a cell’s gene activity. Delivery can support recombinant protein production, transient transfection, stable genetic modification, and functional studies in cultured cells and tissues. These applications let researchers examine gene activity or produce recombinant proteins, while delivery efficiency and expression duration help them interpret experimental outcomes.