Antigen Specific T Cells
Antigen Specific T Cells - Related Videos
03:32
A Technique for the Generation of Antigen-Specific CAR T Cells
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2025
This video demonstrates a technique for generating antigen-specific chimeric antigen receptor (CAR) T cells through retroviral transduction. Activated murine CD8+ T cells are added to a microwell plate coated with recombinant fibronectin fragments. Retroviral vectors containing transgenic RNA encoding an antigen-specific chimeric antigen receptor (CAR) are then introduced, and the plate is centrifuged to facilitate transduction. After incubation, the transduced cells express antigen-specific...
06:20
An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells
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2025
This video demonstrates an assay to analyze antigen-specific CD8+ T cells in different cell cycle stages. Fluorescently-labeled viable cells are selected using flow cytometry, and the cells in the different cell cycle stages are identified by their DNA content using antibodies against cell proliferation-associated nuclear protein and a nucleic acid stain.
10:10
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
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Cited by 6 •
2016
We present here a method to develop functional antigen (Ag)-specific regulatory T cells (Tregs) from induced pluripotent stem cells (iPSCs) for immunotherapy of autoimmune arthritis in a murine model.
05:41
Nanoparticle-Based Enrichment and Expansion of Antigen-Specific CD8+ T Cells
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2025
This video describes a protocol for isolating rare-antigen-specific CD8+ T cells using magnetic nanoparticle-based artificial antigen-presenting cells (aAPCs) functionalized with antigenic peptide-loaded MHC-Igs and anti-CD28 antibodies. Interaction of the aAPCs with CD8+ T cells specifically expressing the antigenic peptide-targeting T cell receptors, followed by their expansion in vitro, results in the enrichment and expansion of this CD8+ T cell population.
09:25
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
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Cited by 36 •
2017
A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.
FAQs
Recognition depends on a precise pairing between a T-cell receptor and an antigen-derived peptide displayed by a major histocompatibility complex molecule on an antigen-presenting cell. This arrangement links the T cell to a particular molecular target rather than activating it indiscriminately. The resulting selectivity helps the adaptive immune system distinguish relevant biological challenges.
Peptide recognition alone does not provide the full activation context described for these lymphocytes. Appropriate co-stimulatory signals help trigger the response associated with clonal expansion, cytokine production, and differentiation. Their role is therefore important because antigen presentation and receptor binding must be accompanied by additional signals before the cells develop the functional outcomes needed for an effective adaptive response.
Activation can produce several linked outcomes: the responsive cells undergo clonal expansion, release cytokines, and differentiate into effector or memory cells. Expansion increases the representation of cells directed toward the recognized target, while differentiation gives the response distinct functional or lasting characteristics. Together, these outcomes connect molecular recognition with active immunity and longer-term protection.
Effector and memory cells represent different outcomes of T-cell differentiation. Effector cells are associated with the active phase of the response, including cytokine production, whereas memory cells support long-term protection after the initial challenge. Studying both populations helps researchers assess not only whether a target-specific response occurred, but also whether it may persist.
Research methods can focus on three related goals: identifying cells directed toward a particular antigen, isolating those cells for further study, or measuring their presence and response. These approaches allow investigators to connect receptor recognition with outcomes such as expansion, cytokine production, or differentiation. The resulting information supports both basic studies of specificity and applied immune monitoring.
Their selective recognition makes them useful indicators of how the adaptive immune system responds to an infection or vaccine-related target. Researchers can study whether target-specific cells are present and examine associated expansion, cytokine production, or memory formation. Such measurements help characterize immune responses and assess the potential for long-term protection without treating all lymphocytes as equivalent.
In cancer research, investigators examine these cells in relation to tumor targets and the development of cancer immunotherapy. In autoimmune disease research, the same principle of molecular specificity helps scientists investigate immune responses directed toward inappropriate biological targets. Identifying, isolating, or measuring the relevant cells can therefore clarify disease-associated immunity and inform studies of selective immune intervention.