Warm Media Recovery
Warm Media Recovery - Related Videos
08:38
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Cited by 14 •
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The goal of this procedure is to easily and rapidly produce a microfluidic device with customizable geometry and resistance to swelling by organic fluids for oil recovery studies. A polydimethylsiloxane mold is first generated, and then used to cast the epoxy-based device. A representative displacement study is reported.
10:00
Gradient Echo Quantum Memory in Warm Atomic Vapor
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The gradient echo memory is a protocol for storing optical quantum states of light in atomic ensembles. Quantum memory is a key element of a quantum repeater, which can extend the range of quantum key distribution. We outline the operation of the scheme when implemented in a 3-level atomic ensemble.
03:36
Recovery of Zika Virus from In Vitro-Transcribed RNA
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2026
Source: Weger-Lucarelli, J., et al. Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone. J. Vis. Exp. (2017)This video demonstrates the recovery of infectious Zika virus using electroporation of in vitro transcribed RNA. The process shows how synthetic RNA initiates infection and leads to viral replication in cultured cells.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
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Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
13:48
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
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Cited by 24 •
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Noroviruses are a major cause of gastroenteritis yet molecular techniques for their characterisation are still relatively new. Here we report two different reverse genetics approaches for the efficient recovery of murine norovirus (MNV), the only member of this genus which can be propagated in cell culture.
FAQs
Prewarming supports stressed cells as they repair damage associated with DNA uptake. The controlled warm environment helps restore membrane function and resume metabolic activity, creating conditions in which cells can remain viable after transformation. Without this recovery opportunity, fewer cells may survive long enough to produce colonies during subsequent selective plating.
Nutrient-rich medium supplies the environment needed for metabolic recovery while cells begin using newly acquired genetic information. In bacterial transformation, this interval is especially important because cells need time to express antibiotic-resistance genes before encountering selective conditions. The resulting expression improves the likelihood that successfully transformed cells will withstand selection.
Recovery increases the number of viable transformed cells available for selection. Rather than exposing cells to selective plating immediately after DNA uptake, the process gives them time to repair membranes, restore metabolism, and express resistance genes. More surviving cells can then form colonies, improving transformation efficiency and providing more material for downstream analysis.
The key conditions identified for this step are a prewarmed growth medium and a controlled recovery environment. These conditions must support viability while cells repair and express newly acquired genes. The source does not specify an exact temperature or recovery duration, so those parameters should not be assumed from the technique name alone.
Following DNA uptake, transformed bacterial cells are transferred into prewarmed, nutrient-rich growth medium. They remain under controlled conditions long enough to recover and express newly acquired antibiotic-resistance genes. The culture is then subjected to selective plating, where resistant cells can form colonies for counting, evaluation, or further analysis.
This recovery step is useful in workflows that introduce plasmids or other genetic changes into microorganisms and then identify successful transformants through selection. It supports bacterial transformation, genetic engineering, and plasmid-based experiments by increasing the number of colonies available for analysis. Its value is greatest when cell stress and gene-expression timing affect selection outcomes.