Biology Lab Techniques
Biology Lab Techniques - Related Videos
04:14
Lab Techniques - Concepts
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2020
Measurement Accuracy The ability to repeat an experiment and get the same results, or reproducibility, is essential in scientific research. However, it’s impossible to repeat an experiment if you don’t know how you did it. Thus, scientists keep detailed records of their experiments in lab notebooks. These records include important information like the amount of each material used and descriptions of each step of the procedure. Equipment, like balances and volumetric or graduated glassware, is...
01:21
Lab Techniques - Instructor Prep
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparation of LaboratoryExpand Here we show the laboratory preparation for 10 students working individually, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Obtain enough small plastic bottles with caps for the number of students and label them ‘NaCl’. Use a...
08:53
Lab Techniques - Student Protocol
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Determining the Density of an EggExpand Density is a measure of how compact a substance or object is, and it is calculated as mass divided by volume. When a dense object is placed in a liquid with a lower density, the object sinks; when the object is less dense than the liquid, it floats. When the densities are the same, the object will be suspended in the liquid. In this experiment, we'll determine the density of an egg by creating a...
10:39
Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module
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Cited by 4 •
2015
A feasible laboratory module for biology undergraduates that explores advanced cellular and molecular concepts using animal cell culture is described. Students grow, characterize and manipulate a breast cancer cell model by exposure to chemotherapy agents. Cell viability is assayed through cell counting using both a standard and novel method.
11:14
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
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Cited by 2 •
2017
We describe a framework incorporating straightforward biochemical and computational analysis to guide the characterization and crystallization of large coiled-coil domains. This framework can be adapted for globular proteins or extended to incorporate a variety of high-throughput techniques.
FAQs
Standardized protocols specify how biological materials are handled, measured, or analyzed so that experiments follow consistent procedures. This consistency allows researchers to compare observations across samples or experiments and helps distinguish genuine biological differences from variation caused by changing methods. As a result, protocols support reproducibility when investigators test mechanisms, compare responses, or validate hypotheses.
Calibrated instruments provide measurements that are adjusted for dependable performance, while controlled variables limit unwanted differences between experimental conditions. Together, they improve the reliability of observations and make biological responses easier to interpret. These controls are especially important when researchers measure changes in cells or biological molecules and need to determine whether those changes support a proposed explanation.
Sterile handling is one component of maintaining controlled laboratory conditions. It helps keep sample preparation and analysis consistent with the intended experimental design, particularly when biological materials are manipulated or prepared for later measurement. Used alongside standardized protocols and calibrated equipment, sterile practice contributes to observations that can be reproduced and meaningfully compared.
A biological workflow may begin by preparing or separating a sample, continue with controlled handling and measurement, and end by detecting changes in biological molecules or cells. Each stage supplies information needed by the next, so consistency throughout the workflow matters. This sequence lets investigators move from a prepared biological material to observations that can be analyzed against a hypothesis.
These techniques support work in genetics, microbiology, physiology, and biotechnology. In each area, researchers can use controlled observations to investigate biological mechanisms, compare responses, or examine changes in cells and molecules. Their broad application reflects the need for practical laboratory methods across fields that study different levels of biological organization and different types of experimental questions.
Laboratory methods can generate observations that help identify mechanisms, compare biological responses, and validate hypotheses. The resulting evidence also supports applied goals, including the development of diagnostic or therapeutic approaches. Their value therefore extends beyond producing measurements: when procedures and variables are controlled, the findings can connect experimental observations with explanations and potential biological or medical applications.