Human Cells
Human Cells - Related Videos
10:33
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Cited by 24 •
2014
Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
08:05
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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Cited by 3 •
2016
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
09:43
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
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Cited by 13 •
2014
Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced efficiency.
11:56
Derivation of Human Embryonic Stem Cells by Immunosurgery
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Cited by 12 •
2007
The ability of human embryonic stem cells to self-renew and differentiate into all cell types of the body suggests that they hold great promise for both medical applications and as a research tool for addressing fundamental questions in development and disease. Here, we provide a concise, step-by-step protocol for the derivation of human embryonic stem cells from embryos by immunosurgical isolation of the inner cell mass.
FAQs
Selective permeability allows the plasma membrane to regulate which substances move between a cell and its surroundings. This controlled exchange helps maintain the internal conditions needed for cellular activity rather than permitting unrestricted movement. Because membrane regulation influences how cells respond to their environment, it is central to understanding cell function, tissue coordination, and homeostasis.
DNA provides the information used to direct gene expression, which determines when particular proteins are produced. These proteins support cellular functions such as metabolism and responses to signals. Differences in gene activity therefore help explain why cells behave differently, even though coordinated genetic regulation remains essential for growth, repair, and maintaining the body's internal balance.
Specialization results from cells regulating distinct gene programs, causing them to develop different functional characteristics. Neurons, muscle cells, and immune cells consequently contribute in different ways to the body's tissues and organs. Their activities also depend on interactions with neighboring cells, so specialization and cell-to-cell coordination work together to support organized biological functions.
Individual cell activities become biologically meaningful when cells interact within tissues. These interactions allow specialized cells to contribute collectively to organ structure, function, growth, and repair. They also connect cellular behavior with homeostasis, the maintenance of stable internal conditions. Studying cells in their tissue context can therefore reveal relationships that isolated cellular observations may not capture.
Researchers examine human cells in several complementary settings, including cell culture, tissues, and model systems. Culture can support focused investigation of cellular behavior, whereas tissues provide context for interactions among specialized cells. Model systems extend these investigations to broader questions about development and disease. Comparing these settings helps connect cellular mechanisms with organized biological outcomes.
Human-cell research can clarify how normal development and disease relate to cellular activities, gene regulation, signaling, and interactions within tissues. It also supports evaluation of drug effects and investigation of therapeutic strategies. In regenerative medicine, these studies are relevant to understanding how cellular processes connected with growth and repair might contribute to restoring damaged tissues.