Carbon-14 Glucose
Carbon-14 Glucose - Related Videos
05:52
In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement
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2025
This video demonstrates an in vitro assay to indirectly measure intracellular glucose uptake. The assay uses a fluorescently-labeled glucose analog to establish a correlation between extracellular glucose depletion and intracellular glucose uptake.
02:23
Oral Glucose Tolerance Test (OGTT): Measuring Glucose Metabolism in Mice
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2023
This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.
11:04
NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
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2025
The primary objective of this article is to help researchers and scientists understand the process of glucose sensing using electrochemistry and to optimize parameters to maximize the glucose sensing response. The process of fabricating a glucose sensor is described in detail with guidelines for obtaining its best chronoamperometric response.
04:11
Assessing Glucose Uptake in the Motor Neurons of Drosophila Larvae with Upregulated Glucose Metabolism
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2025
The video demonstrates a technique for measuring glucose uptake in Drosophila larval motor neurons using a fluorescence resonance energy transfer (FRET)-based intracellular glucose sensor. After dissecting the larvae to expose the ventral nerve cord, the motor neurons are visualized through the fluorescence emitted by the sensor's donor fluorophore. When stimulated with glucose, FRET between the sensor's donor and acceptor fluorophores triggers the emission of fluorescence from the acceptor,...
01:14
The Carbon Cycle
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2019
Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle. Biological Carbon Cycle All...
FAQs
Once cells take up the labeled molecule, carbon-14 can appear in downstream metabolites, newly formed biomass, or released carbon dioxide. Comparing where the isotope accumulates shows how much glucose carbon enters glycolysis, cellular respiration, or biosynthetic routes. This connects tracer location with pathway activity rather than merely showing that glucose was present.
Measurements can separate the amount of labeled glucose entering a biological system from the subsequent processing of that glucose. Carbon-14 detected in cells or metabolites indicates uptake and retention, while its appearance in downstream products or carbon dioxide indicates metabolic conversion. This distinction helps researchers evaluate both transport into cells and pathway use.
These destinations represent different outcomes for glucose carbon. Labeled metabolites indicate processing through intermediate steps, biomass shows incorporation into cellular material, and carbon dioxide reflects carbon released during cellular respiration. Examining them separately helps determine whether glucose primarily supports pathway activity, biosynthesis, or energy production in the studied biological system.
Researchers introduce the labeled glucose to cells, microbes, tissues, or another biological system and allow normal glucose processing to occur. They then follow carbon-14 in relevant metabolites, biomass, or released carbon dioxide and measure the label through radiometric detection. The resulting distribution provides evidence about glucose uptake, metabolic flux, and carbon use.
The tracer is useful when researchers need to follow carbohydrate movement or determine how glucose contributes to cellular activity. Applications described for biology include studies of cell physiology, microbial metabolism, tissue function, and disease-related metabolic changes. It can reveal differences in glucose uptake, pathway use, or biosynthetic incorporation across biological conditions.
Carbon-14 emits ionizing radiation, so experiments require careful handling of the labeled material and the biological samples associated with it. This consideration affects laboratory planning as well as experimental execution. Researchers must preserve the ability to detect the tracer while treating the isotope as a radioactive substance throughout the study.