Cartilage Explants
Cartilage Explants - Related Videos
07:58
Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants
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Cited by 5 •
2021
Here, we present a protocol to label platelet lysate-derived extracellular vesicles to monitor their migration and uptake in cartilage explants used as a model for osteoarthritis.
07:10
An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
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Cited by 7 •
2019
Here, we present a protocol describing isolation and culturing of cartilage explants from bovine knees. This method provides an easy and accessible tool to describe tissue changes in response to biological stimuli or novel therapeutics targeting the joint.
06:42
In Vitro Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants
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2025
To better understand the chondrocyte injury response to a mechanical cartilage overload, the protocol describes the development of an ex vivo cartilage impact model that was sublethal for 24 h.
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
FAQs
Cartilage explants preserve resident chondrocytes within their native extracellular matrix, so observations include interactions between cells and their surrounding scaffold. This context can reveal changes in tissue structure and integrity that isolated-cell systems may not capture as effectively. The approach is therefore useful when the research question concerns how cartilage tissue, rather than chondrocytes alone, responds to an intervention.
Researchers can alter nutrient conditions, mechanical loading, inflammatory stimuli, or drug exposure while maintaining the tissue outside the body. These variables allow investigators to examine how controlled challenges affect matrix composition, cell viability, and overall tissue integrity. Comparing responses across conditions helps connect a specific experimental input with tissue-level changes in cartilage biology.
Mechanical loading and inflammatory stimuli provide distinct experimental challenges for cartilage tissue. By applying these conditions to cultured explants, investigators can assess resulting changes in matrix composition, chondrocyte viability, and tissue integrity. This makes the model useful for connecting biologically relevant stresses with tissue responses in studies of cartilage biology, osteoarthritis, and injury.
Researchers maintain the explants outside the body, establish the chosen nutrient or loading conditions, and introduce inflammatory stimuli or candidate drugs when appropriate. They then measure matrix composition, cell viability, and tissue integrity. This sequence links controlled culture conditions to measurable tissue outcomes without removing resident chondrocytes from their native extracellular matrix.
Researchers use cartilage explants when they need a tissue-level model for examining osteoarthritis, injury, or repair. Because the system preserves cell-matrix interactions while permitting controlled experimental exposure, it can show how cartilage responds as an intact tissue. Findings may help characterize disease-related changes or assess repair-oriented interventions.
Cartilage explants provide a controlled testing context for exposing cartilage tissue to biomaterials or candidate treatments. Researchers can measure matrix composition, cell viability, and tissue integrity after exposure to determine how the intervention affects the tissue. These outcomes can inform evaluation before progression to animal studies or eventual clinical translation.