Cytotoxicity Screening
Cytotoxicity Screening - Related Videos
09:57
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
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Cited by 15 •
2017
We have developed a modular high-throughput screening system for discovering novel compounds against Mycobacterium tuberculosis, targeting intracellular and in-broth growing bacteria as well as cytotoxicity against the mammalian host cell.
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
03:01
alamarBlue Assay: A Sensitive Fluorometric Method to Screen the Cytotoxic Effects of Artificial Tear Formulations on Metabolic Activity of Human Corneal Epithelial Cells
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2025
This video describes an in vitro fluorometric assay to assess the effects of artificial tear formulations on the cellular metabolic activity of corneal epithelial cells using resazurin-based Alamar blue solution. A non-toxic formulation maintains the cells in a healthy, metabolically active state, facilitating the reduction of resazurin into a highly fluorescent resorufin.
13:59
High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds
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Cited by 2 •
2023
This protocol presents an automated, image-based high-throughput technique to identify compounds modulating natural killer cell-mediated breast cancer cell killing in the presence of a therapeutic anti-HER-2 antibody.
03:00
An Assay to Evaluate Immunodominance in Cytotoxic T Lymphocyte Responses
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2025
The video showcases a flow cytometry-based assay for assaying immunodominance in cytotoxic T lymphocytes or CTL responses. Injected fluorescent target cells exhibit varying fluorescence corresponding to immunogenic epitopes. Post-spleen collection and sorting reveal a reduction in high-intensity fluorescent cells, confirming immunodominance in CTL responses.
FAQs
The measured endpoint determines which cellular change is being assessed. Metabolic activity can indicate whether cells remain functionally active, while membrane integrity reflects damage to the cell boundary. Measurements of cell proliferation or apoptosis provide additional perspectives on whether treatment limits cell growth or activates a regulated cell-death process. Together, these readouts help characterize cellular injury more precisely.
Dose-response relationships show how cellular effects change as the concentration of a substance increases. Researchers can use these patterns to compare toxic effects across treatments and identify concentrations associated with reduced viability. Measures such as inhibitory concentration summarize part of this relationship, supporting comparisons among compounds, exposure levels, or experimental conditions.
These measurements describe different consequences of exposure rather than a single universal response. Reduced metabolic activity may indicate declining cellular function, altered membrane integrity signals structural damage, and apoptosis measurements identify a cell-death response. Examining these outcomes separately can help researchers characterize the nature of injury and relate it to the tested substance.
A typical experiment uses cultured cells, exposes them to defined concentrations of a compound, and measures a selected indicator of viability. The investigator may assess metabolic activity, membrane integrity, cell proliferation, or apoptosis, then compare the results across concentrations. Dose-response analysis and inhibitory concentration measures help organize and interpret the observed effects.
During therapeutic research, screening helps identify candidates that produce harmful effects on cultured cells before more advanced studies. It can also support investigation of how a promising candidate causes cellular injury. By comparing viability-related outcomes across treatments and concentrations, researchers can distinguish candidates requiring further evaluation from those showing concerning toxicity.
For environmental exposure studies, the assays help evaluate whether a substance harms cultured cells at defined concentrations. In biomaterials research, they provide a way to examine whether a material or its associated exposure produces damaging cellular effects. These results contribute to safety evaluation alongside applications in drug research and biological investigation.