Electroporation Transfection
Electroporation Transfection - Related Videos
15:56
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
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Cited by 99 •
2009
We describe detailed procedures for the efficient transfection of plasmid DNA into the fibers of foot muscles of live mice using electroporation and the subsequent visualization of protein expression using fluorescence microscopy.
12:55
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
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Cited by 11 •
2010
This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.
05:26
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Cited by 5 •
2011
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.
09:30
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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Cited by 49 •
2011
A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.
05:11
Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids
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2023
This video describes the electroporation protocol for transfecting large plasmids into three-dimensional gastrointestinal organoids to prove its universal functionality in different entities, namely pancreatic ductal adenocarcinoma (PDAC), colorectal cancer (CRC), cholangiocarcinoma (CCC), and gastric cancer (GC) organoids.
FAQs
The electrical pulse must create membrane pores large or persistent enough for nucleic acid entry without causing excessive cellular damage. Increasing pulse strength or duration can change delivery efficiency, while cell condition also influences viability. Researchers therefore balance these variables to obtain useful molecular delivery while preserving enough living cells for downstream gene-expression or functional studies.
Cells do not respond identically to the same electrical treatment, so their condition is an important determinant of both delivery and survival. Healthy, suitable cells can support membrane resealing and subsequent analysis more effectively than compromised cells. Accounting for cell condition helps researchers interpret differences in nucleic acid uptake, gene expression, and experimental viability.
Electroporation transfection provides an alternative when cells are difficult to modify with chemical transfection. Its electrical mechanism can support nucleic acid delivery in cell types that resist chemical approaches, expanding the range of biological systems available for study. The central experimental consideration remains the balance between effective cargo entry and preservation of viable cells.
The method can introduce DNA, RNA, and related molecular cargo into living cells. Depending on the experimental design, this delivery can produce transient gene expression, alter cellular function, or support genome-editing studies. These outcomes allow investigators to connect introduced nucleic acids with observable cellular changes and to examine gene activity in controlled biological experiments.
A basic workflow involves preparing living cells and the selected nucleic acid cargo, applying controlled electrical pulses, and allowing the membrane to reseal before evaluating the result. Researchers then examine delivery-related outcomes such as gene expression or altered cellular function, while also considering cell viability. Pulse strength, duration, and cell condition are adjusted during optimization.
Researchers may select this approach for transient gene-expression experiments, genome editing, functional genomics, or studies requiring manipulation of cells that resist chemical transfection. It is also relevant to developing and optimizing nucleic acid delivery systems. By enabling controlled molecular changes in living cells, the technique supports investigations of cellular function and experimental gene regulation.