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Neuroscience

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Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
 

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Article DOI: 10.3791/2678
April 17th, 2011

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Summary April 17th, 2011

We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.

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