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Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Neuroscience
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JoVE Journal Neuroscience
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

DOI:
10.3791/2678-v

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05:26 min

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April 17, 2011

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1Department of Neurobiology,Yale University, 2Program in Developmental Biology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:24Equipment Set-up for in vivo Electroporation
  • 02:08Micropipette and Injector Set-up for Retinal Microinjection
  • 02:44Retinal Injection and Electroporation
  • 04:06Single Retinal Ganglion Cell Labeling with EGFP Plasmid Constructs
  • 05:00Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.

Tags

TransfectionMouse Retinal Ganglion CellsIn Vivo ElectroporationRGC ProjectionsNeural ConnectivityRetinofugal ProjectionsLateral Geniculate NucleusSuperior ColliculusDevelopmental ChangesAxonal Arbor MorphologyRetinotopic Map FormationGenetic ManipulationElectroporation ProtocolsPostnatal MiceLabeling Efficiency

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