Journal
/
Biology
/
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

DOI:
10.3791/1662-v

•

12:55 min

•

January 07, 2010

•

, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc.

Chapters

  • 00:00Title
  • 00:44Introduction
  • 02:07Preparing the Mouse Embryonic Fibroblasts
  • 04:00The 96-well Electroporation Plates
  • 05:20Electroporating the MEFs in 96-well Plates
  • 07:49Electroporating the MEFs in Cuvettes
  • 10:11Epifluorescence Microscopy Images of Transfected Cells
  • 12:26Conclusion

Summary

Automatic Translation

Automatic Translation

This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.

Tags

Gene Pulser MXcell Electroporation SystemTransfectPrimary CellsHigh EfficiencyPlasmid DNASiRNAElectroporation BufferBio-RadNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsElectroporation ConditionsOptimization96-well Electroporation PlatesElectroporation CuvettesElectroporation EfficiencySuccess Or Failure Of Electroporation Experiments

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1662?article_type=v

Electroporation of Mycobacteria
check_url/1662?article_type=v

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
check_url/1662?article_type=v

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
check_url/1662?article_type=v

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
check_url/1662?article_type=v

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
check_url/1662?article_type=v

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
check_url/1662?article_type=v

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
check_url/1662?article_type=v

Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva
check_url/1662?article_type=v

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
check_url/1662?article_type=v

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Read Article