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11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
0 Views •
Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
06:03
A Push-pull Protocol to Reduce Colonization of Bird Nest Boxes by Honey Bees
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Cited by 1 •
2016
Preventing colonization of bird nest boxes by invasive Africanized honey bees is important for conservation efforts of nest-site limited birds. We provide an integrated pest management approach to "push" bees away from nest boxes with a repellant insecticide, permethrin, and "pull" them toward pheromone baited swarm traps.
12:07
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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Cited by 40 •
2012
Reactive oxygen species level is elevated when cells encounter stress conditions. Here we show the example of 3'-3' diaminobenzidine staining as well as cysTMT labeling and mass spectrometry to profile the redox proteome in Pseudomonas syringae treated tomato leaves.
09:00
Cargo Loading onto Kinesin Powered Molecular Shuttles
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Cited by 4 •
2010
Molecular shuttles consisting of functionalized microtubules gliding on surface-adhered kinesin motor proteins can serve as a nanoscale transport system. Here, the assembly of a typical shuttle system is described.
08:49
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
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Cited by 2 •
2015
This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.
FAQs
Fragmented habitat can restrict movement between areas used for hunting, reproduction, and survival. When populations become isolated, individuals have fewer opportunities to move between groups, which can limit gene flow. Reduced gene flow may lower genetic diversity, making habitat connectivity an important biological consideration in conservation planning for Florida panthers.
Genetic diversity reflects the range of inherited variation within a population. For Florida panthers, habitat isolation can reduce gene flow and therefore contribute to lower genetic diversity. Genetic monitoring helps biologists evaluate this condition and determine when conservation strategies should address population connectivity or other aspects of genetic management.
As carnivorous mammals, Florida panthers influence the abundance and distribution of prey through predation. This role can affect relationships within southern Florida ecosystems, linking the panther’s conservation to broader ecological function. Protecting the population therefore supports not only an endangered animal but also the ecosystem processes associated with its position as a predator.
Biologists combine field observations, radio telemetry, population surveys, and genetic monitoring rather than relying on a single source of evidence. Together, these approaches provide information about health, behavior, population size, and genetic condition. Using several forms of monitoring gives conservation planning a broader biological basis for evaluating population status and change.
Radio telemetry is one of the field-based tools used to study Florida panthers. In the broader monitoring program, it contributes evidence about the animals alongside direct observations, population surveys, and genetic data. Such complementary information helps biologists examine behavior and habitat-related needs while assessing conditions that influence movement, reproduction, and population recovery.
Monitoring results guide practical strategies including habitat protection, wildlife corridors, and genetic-management efforts. Habitat protection preserves areas needed by the population, while corridors can improve connections among suitable habitats. Genetic-management strategies address concerns about reduced diversity. Together, these applications translate biological evidence into actions intended to support recovery of the endangered population.