High Pressure Valve

A high pressure valve is a mechanical device that controls, directs, or stops fluid flow while containing elevated pressures, making it essential for safely managing gases and liquids in laboratory and industrial systems. It operates by moving a plug, diaphragm, ball, or other sealing element against a seat; an actuator or pressure difference changes the opening, while the valve body and seals withstand the applied load and prevent leakage. In biology, high pressure valves support bioprocessing, high-pressure sterilization, fluid transfer, and instrumentation by maintaining controlled conditions, protecting samples and equipment, and enabling reproducible experiments involving cells, proteins, or microorganisms.

High Pressure Valve - Related Videos

Research

JoVE EoE - Rodent Models

Pressure Fixation of Isolated Murine Pulmonary Valve: A Procedure to Isolate and Chemically Fix the Mouse Pulmonary Valve in Closed Conformation

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2025

In this video, we describe the procedure to isolate and chemically fix the mouse pulmonary value in closed conformation via perfusion pressurization.

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

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Cited by 7 •

2011

A cyclic pressure bioreactor capable of subjecting heart valve tissue to physiological and pathological pressure conditions has been designed. A LabVIEW program allows users to control pressure magnitude, amplitude and frequency. This device can be used to study the mechanobiology of heart valve tissue or isolated cells.

Synthesis and Microdiffraction at Extreme Pressures and Temperatures

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Cited by 5 •

2013

The laser heated diamond anvil cell combined with synchrotron micro-diffraction techniques allows researchers to explore the nature and properties of new phases of matter at extreme pressure and temperature (PT) conditions. Heterogeneous samples can be characterized in situ under high pressure by 2D mapping and combined powder, single-crystal and multigrain diffraction approaches.

Ammonia Synthesis at Low Pressure

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Cited by 17 •

2017

Ammonia can be synthesized at low pressure by using a conventional catalyst and an ammonia selective absorbent.

Research

JoVE Journal - Developmental Biology
Free Sample

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

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Cited by 16 •

2017

This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.

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