Il-17 Secretion Assay
Il-17 Secretion Assay - Related Videos
12:38
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
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2008
This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.
11:53
ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
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2023
Source: Tonya J. Webb1 1 Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center, Baltimore, Maryland 21201 ELISPOT is a standardized, reproducible assay used to detect cellular immune responses. The assay utilizes an enzyme-linked immunosorbent assay (ELISA)- based method to detect single-cell immune responses which can be visualized by spots, hence the name ELISPOT. ELISPOT was first described in...
04:20
Functional Assessment of Antibody-Secreting B Cells Using ELISpot Assay
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2025
This video demonstrates the use of the ELISpot assay to assess the function of antibody-secreting B cells, ASCs. The antibodies secreted by the ASCs interact with the membrane-bound polyclonal antibody fragments, which are detected by the enzyme-conjugated IgG or IgM antibodies. The colored spot produced in each well indicates the presence of the specific antibody's isotype, which correlates with the B cells' secretory activity.
05:28
Assay for the Identification of Interferon-Gamma Secretion in Murine Lymphoid Organs
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2025
This video demonstrates an imaging method to visualize in situ interferon-gamma (IFN-γ) secretion in the mouse spleen. Following bacterial infection and the induction of IFN-γ accumulation inside T cells and natural killer cells, the spleen is harvested from the mouse. Upon obtaining tissue sections, immunostaining is performed to visualize IFN-γ inside the cells.
13:30
Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule
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Cited by 23 •
2015
This protocol describes the use of the Ramsay assay to measure fluid secretion rates from isolated Malpighian (renal) tubules from Drosophila melanogaster. In addition, the use of ion-specific electrodes to measure sodium and potassium concentrations in the secreted fluid, allowing calculation of transepithelial ion flux, is described.
FAQs
Signal strength is interpreted as a proxy for the amount of IL-17 released: more captured cytokine produces a stronger enzyme-linked or fluorescence-based detection signal. Because the assay measures secretion after cells are activated under defined conditions, comparisons are most meaningful when the same activation and measurement conditions are maintained across samples.
The capture antibody provides the specificity that distinguishes IL-17 from other substances released by activated cells. After secretion, it binds the target cytokine, and the assay's enzyme-linked or fluorescence-based detection system converts that binding event into a measurable signal. This design connects molecular recognition with quantitative comparison of immune-cell responses.
Activation is not merely a preliminary step; it creates the experimental context in which cytokine release is assessed. Defined activation conditions allow researchers to compare how isolated cells respond and to examine pathways associated with inflammation and host defense. Changing the biological context can therefore alter the IL-17 measurement and its interpretation.
A typical workflow begins with isolated immune cells, followed by activation under defined conditions. The released material is exposed to an IL-17-specific capture antibody, and the bound cytokine is detected through an enzyme-linked or fluorescence-based readout. The resulting signal is then used to compare secretion among cellular conditions or experimental groups.
Researchers can use the assay to characterize immune-cell function by comparing IL-17 secretion between samples. The measurement is especially informative when the goal is to determine whether cellular responses differ under the tested conditions, rather than simply to identify the presence of immune cells. Results can support analysis of inflammatory and host-defense responses in biology.
Within biology, IL-17 secretion measurements provide a readout for studies of autoimmune disease, infection, immunotherapy, and other inflammatory processes. The assay can help connect immune-cell activity with biological outcomes by showing how much cytokine is released under defined conditions. Interpretation remains centered on secretion, so the result complements broader investigation of relevant pathways and responses.