Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Detection and Isolation of Viable Mouse…
JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
Journal (Biology)
Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
Journal (Biology)
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
 

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Article DOI: 10.3791/1037 • 12:39 min • December 18th, 2008
December 18th, 2008 •
1, 1, 1, 1, 1, 1
1Miltenyi Biotec,GmbH

Chapters

Summary
Automatic Translation

This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.

Tags

Detection Isolation Viable Mouse IL-17-secreting T Cells MACS Cytokine Secretion Assay Single Cell Level Sensitive Isolation Cytokine-secreting Cells IL-17-secreting Cells Single Cell Suspension Mouse Splenocytes PMA/ionomycin Cytokine Secretion IL-17 Catch Reagent Bi-specific Antibody CD45 Leukocytes IL-17 Secretion Restart Temperature Increase Trapped IL-17 Flow Cytometry Anti-Biotin-PE Antibody
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter